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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
1998-6-3
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pubmed:abstractText |
IL-10 has a well-characterized anti-inflammatory role that includes the suppression of inflammatory cytokine (e.g. TNF-alpha) production by monocytic/macrophage cells. Both transcriptional and post-transcriptional/translational mechanisms have been proposed to explain this process. In this study we observed that IL-10 inhibited nuclear NF kappa B DNA binding activity without affecting I kappa B degradation or translocation of NF kappa B subunits to the nucleus. While the suppression of NF kappa B in 70Z/3 pre-B cells correlated with suppression of NF kappa B transcriptional activity and expression of surface IgM, it did not correlate with the production of TNF-alpha mRNA or protein in RAW 264.7 macrophages. Similar observations in the macrophages were made with a second anti-inflammatory cytokine, IL-4. Therefore we conclude that although IL-10 or IL-4 can suppress NF kappa B activity, this appears to have little effect on the expression of the TNF-alpha gene and is unlikely to be the basis of the anti-inflammatory effects of these cytokines.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/I kappa B beta protein,
http://linkedlifedata.com/resource/pubmed/chemical/I-kappa B Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Immunosuppressive Agents,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-10,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-4,
http://linkedlifedata.com/resource/pubmed/chemical/Lipopolysaccharides,
http://linkedlifedata.com/resource/pubmed/chemical/NF-kappa B,
http://linkedlifedata.com/resource/pubmed/chemical/NF-kappa B p50 Subunit,
http://linkedlifedata.com/resource/pubmed/chemical/NF-kappaB inhibitor alpha,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factor RelA,
http://linkedlifedata.com/resource/pubmed/chemical/Tumor Necrosis Factor-alpha
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0014-2980
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
28
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1719-26
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pubmed:dateRevised |
2005-11-17
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pubmed:meshHeading |
pubmed-meshheading:9603479-Animals,
pubmed-meshheading:9603479-Biological Transport,
pubmed-meshheading:9603479-Cell Line,
pubmed-meshheading:9603479-Cell Nucleus,
pubmed-meshheading:9603479-DNA,
pubmed-meshheading:9603479-DNA-Binding Proteins,
pubmed-meshheading:9603479-I-kappa B Proteins,
pubmed-meshheading:9603479-Immunosuppressive Agents,
pubmed-meshheading:9603479-Interleukin-10,
pubmed-meshheading:9603479-Interleukin-4,
pubmed-meshheading:9603479-Lipopolysaccharides,
pubmed-meshheading:9603479-Macrophages,
pubmed-meshheading:9603479-Mice,
pubmed-meshheading:9603479-NF-kappa B,
pubmed-meshheading:9603479-NF-kappa B p50 Subunit,
pubmed-meshheading:9603479-Protein Binding,
pubmed-meshheading:9603479-RNA, Messenger,
pubmed-meshheading:9603479-Transcription, Genetic,
pubmed-meshheading:9603479-Transcription Factor RelA,
pubmed-meshheading:9603479-Tumor Necrosis Factor-alpha
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pubmed:year |
1998
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pubmed:articleTitle |
IL-10-mediated suppression of TNF-alpha production is independent of its ability to inhibit NF kappa B activity.
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pubmed:affiliation |
Kennedy Institute of Rheumatology, Hammersmith, London, GB.
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pubmed:publicationType |
Journal Article
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