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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
1998-8-24
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pubmed:abstractText |
To construct a mammalian artificial chromosome (MAC), telomere repeats and selectable markers were introduced into a 100 kb yeast artificial chromosome (YAC) containing human centromeric DNA. This YAC, which has a regular repeat structure of alpha-satellite DNA and centromere protein B (CENP-B) boxes, efficiently formed MACs that segregated accurately and bound CENP-B, CENP-C, and CENP-E. The MACs appear to be about 1-5 Mb in size and contain YAC multimers. Structural analyses suggest that the MACs have not acquired host sequences and were formed by a de novo mechanism. The accurate segregation of the MACs suggests they have potential as vectors for introducing genes into mammals.
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pubmed:commentsCorrections |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Autoantigens,
http://linkedlifedata.com/resource/pubmed/chemical/CENPB protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Centromere Protein B,
http://linkedlifedata.com/resource/pubmed/chemical/Chromosomal Proteins, Non-Histone,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Satellite,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/centromere protein C,
http://linkedlifedata.com/resource/pubmed/chemical/centromere protein E
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
1087-0156
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
16
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
431-9
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:9592390-Animals,
pubmed-meshheading:9592390-Autoantigens,
pubmed-meshheading:9592390-Cell Line,
pubmed-meshheading:9592390-Cell Line, Transformed,
pubmed-meshheading:9592390-Centromere,
pubmed-meshheading:9592390-Centromere Protein B,
pubmed-meshheading:9592390-Chromosomal Proteins, Non-Histone,
pubmed-meshheading:9592390-Chromosomes,
pubmed-meshheading:9592390-Chromosomes, Artificial, Yeast,
pubmed-meshheading:9592390-Chromosomes, Human, Pair 21,
pubmed-meshheading:9592390-Cloning, Molecular,
pubmed-meshheading:9592390-DNA, Satellite,
pubmed-meshheading:9592390-DNA-Binding Proteins,
pubmed-meshheading:9592390-Genetic Vectors,
pubmed-meshheading:9592390-Humans,
pubmed-meshheading:9592390-In Situ Hybridization, Fluorescence,
pubmed-meshheading:9592390-Kinetochores,
pubmed-meshheading:9592390-Polymerase Chain Reaction,
pubmed-meshheading:9592390-Telomere,
pubmed-meshheading:9592390-Transfection
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pubmed:year |
1998
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pubmed:articleTitle |
Construction of YAC-based mammalian artificial chromosomes.
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pubmed:affiliation |
Department of Molecular Biology, School of Science, Nagoya University, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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