Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
11
pubmed:dateCreated
1998-7-20
pubmed:abstractText
Hepatocyte nuclear factor 4 (HNF-4) is an orphan intracellular receptor that appears to be a key factor in the regulation of energy metabolism. In order to gain greater understanding of the binding and activation requirements of HNF-4, we performed genetic analysis of the apoCIII promoter, a promoter that has previously been shown to be highly sensitive to HNF-4-induced transcription. We identified two elements within the apoCIII promoter that bind HNF-4, either of which are sufficient to confer promoter induction in response to HNF-4. These two elements are both direct repeat-like in nature, but they differ significantly in motif sequence and the repeats are separated by either 1 or 2 nt. Therefore, to better define the DNA sequence recognition requirements of HNF-4, we utilized PCR-based binding site selection. HNF-4 selected for direct repeat-like elements with either 1 or 2 nt between the repeats. Surprisingly, the strongest selection was for a core motif that included the nucleotides between the repeats. Mutation of the nucleotide between the repeats resulted in a 6-fold reduction in affinity, indicating that the interaction between HNF-4 and the intervening nucleotide(s) is critical for high affinity binding.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/9592157-1312668, http://linkedlifedata.com/resource/pubmed/commentcorrection/9592157-1618160, http://linkedlifedata.com/resource/pubmed/commentcorrection/9592157-1639815, http://linkedlifedata.com/resource/pubmed/commentcorrection/9592157-1734282, http://linkedlifedata.com/resource/pubmed/commentcorrection/9592157-2279702, http://linkedlifedata.com/resource/pubmed/commentcorrection/9592157-2744487, http://linkedlifedata.com/resource/pubmed/commentcorrection/9592157-7615825, http://linkedlifedata.com/resource/pubmed/commentcorrection/9592157-7650015, http://linkedlifedata.com/resource/pubmed/commentcorrection/9592157-7651430, http://linkedlifedata.com/resource/pubmed/commentcorrection/9592157-7926749, http://linkedlifedata.com/resource/pubmed/commentcorrection/9592157-7979245, http://linkedlifedata.com/resource/pubmed/commentcorrection/9592157-7984419, http://linkedlifedata.com/resource/pubmed/commentcorrection/9592157-8132632, http://linkedlifedata.com/resource/pubmed/commentcorrection/9592157-8344962, http://linkedlifedata.com/resource/pubmed/commentcorrection/9592157-8366086, http://linkedlifedata.com/resource/pubmed/commentcorrection/9592157-8392479, http://linkedlifedata.com/resource/pubmed/commentcorrection/9592157-8521508, http://linkedlifedata.com/resource/pubmed/commentcorrection/9592157-8521510, http://linkedlifedata.com/resource/pubmed/commentcorrection/9592157-8945471, http://linkedlifedata.com/resource/pubmed/commentcorrection/9592157-8995295, http://linkedlifedata.com/resource/pubmed/commentcorrection/9592157-9153249, http://linkedlifedata.com/resource/pubmed/commentcorrection/9592157-9371825, http://linkedlifedata.com/resource/pubmed/commentcorrection/9592157-942051
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0305-1048
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
26
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2702-7
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1998
pubmed:articleTitle
DNA binding and transcription activation specificity of hepatocyte nuclear factor 4.
pubmed:affiliation
Ligand Pharmaceuticals Inc., 10255 Science Center Drive, San Diego, CA 92121, USA. jfraser@ligand.com
pubmed:publicationType
Journal Article