Linked Life Data

9592099

Source:http://linkedlifedata.com/resource/pubmed/id/9592099

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
11
pubmed:dateCreated
1998-6-4
pubmed:abstractText
Basal forebrain cholinergic neurons (BFCNs) provide the major subcortical source of cholinergic input to cerebral cortex and play an important role in regulating cortical activity. The present study examined the ability of BFCNs to influence neocortical neuronal growth by examining effects of the presence of BFCNs on certain cortical neurons grown under the controlled conditions of dissociated cell culture. Initial experiments demonstrated distinctive morphological features of a population of neurons (labeled with SMI-32, a monoclonal antibody to nonphosphorylated neurofilament proteins that labels pyramidal neurons in vivo) in cocultures containing basal forebrain (BF) and cortical cells. These neurons (large neurons immunoreactive for SMI-32 [SMI-32(+) neurons]) were characterized as having extensive axons, greater soma size, and more dendritic growth than did most SMI-32(+) neurons in the cultures. Staining for SMI-32 in cocultures in which the cortical neurons were labeled with a fluorescent marker before adding the BF cells indicated that virtually all large SMI-32(+) neurons were of cortical origin. Eliminating BFCNs with the selective cholinergic immunotoxin 192 IgG-saporin resulted in a >80% decrease in the number of large SMI-32(+) neurons, although causing little damage to other cells in the treated cultures; this suggests that survival or maintenance of large SMI-32(+) neurons may depend on ongoing trophic support from BFCNs. Thus, present findings suggest that BFCNs may provide powerful growth- and/or survival-enhancing signals to a subset of cortical neurons.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0270-6474
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
18
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
4201-15
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed-meshheading:9592099-Animals, pubmed-meshheading:9592099-Antibodies, Monoclonal, pubmed-meshheading:9592099-Cell Size, pubmed-meshheading:9592099-Cells, Cultured, pubmed-meshheading:9592099-Cerebral Cortex, pubmed-meshheading:9592099-Choline O-Acetyltransferase, pubmed-meshheading:9592099-Cholinergic Agents, pubmed-meshheading:9592099-Cholinergic Fibers, pubmed-meshheading:9592099-Female, pubmed-meshheading:9592099-Immunotoxins, pubmed-meshheading:9592099-N-Glycosyl Hydrolases, pubmed-meshheading:9592099-Neurofilament Proteins, pubmed-meshheading:9592099-Phenotype, pubmed-meshheading:9592099-Pregnancy, pubmed-meshheading:9592099-Pyramidal Cells, pubmed-meshheading:9592099-Rats, pubmed-meshheading:9592099-Rats, Sprague-Dawley, pubmed-meshheading:9592099-Ribosome Inactivating Proteins, Type 1, pubmed-meshheading:9592099-Substantia Innominata
pubmed:year
1998
pubmed:articleTitle
Distinctive morphological features of a subset of cortical neurons grown in the presence of basal forebrain neurons in vitro.
pubmed:affiliation
Department of Anatomy and Neurobiology, University of California, Irvine, Irvine, California 92697-4292, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't