Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1998-6-2
pubmed:abstractText
This study describes the novel localization of the antimicrobial peptide human intestinal defensin-5 (HD-5) in female genital tract epithelia. Using a 3' rapid amplification of cDNA ends (RACE) protocol, HD-5 was cloned from a vaginal epithelial cell RNA preparation, and its identity was confirmed by sequencing. Tissue samples from multiple donors were subsequently screened for HD-5 expression by reverse transcription polymerase chain reaction. HD-5 message was invariantly expressed by normal vagina and ectocervix and inflamed fallopian tube, but variably expressed by normal endocervix, endometrium, and fallopian tube (60, 64, and 29% of specimens, respectively). Expression in endometrium was the highest during the early secretory phase of the menstrual cycle. Using immunohistochemistry and confocal microscopy, HD-5 peptide was localized in the upper half of the stratified squamous epithelium of the vagina and ectocervix, with the intensity of cellular staining increasing toward the lumen. In positive endocervix, endometrium, and fallopian tube specimens, HD-5 was located in apically oriented granules and on the apical surface of a proportion of columnar epithelial cells. Using Western blot analysis, secreted HD-5 was detected in cervicovaginal lavages, with the highest concentrations found during the secretory phase of the menstrual cycle. We hypothesize that HD-5 is an intrinsic component of the female urogenital innate immune defense system and that its expression may be modulated by hormonal and proinflammatory factors.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/9588893-10328022, http://linkedlifedata.com/resource/pubmed/commentcorrection/9588893-1167179, http://linkedlifedata.com/resource/pubmed/commentcorrection/9588893-1339298, http://linkedlifedata.com/resource/pubmed/commentcorrection/9588893-1429669, http://linkedlifedata.com/resource/pubmed/commentcorrection/9588893-1500431, http://linkedlifedata.com/resource/pubmed/commentcorrection/9588893-2023943, http://linkedlifedata.com/resource/pubmed/commentcorrection/9588893-2040802, http://linkedlifedata.com/resource/pubmed/commentcorrection/9588893-2571599, http://linkedlifedata.com/resource/pubmed/commentcorrection/9588893-2715173, http://linkedlifedata.com/resource/pubmed/commentcorrection/9588893-2997278, http://linkedlifedata.com/resource/pubmed/commentcorrection/9588893-3023659, http://linkedlifedata.com/resource/pubmed/commentcorrection/9588893-3120589, http://linkedlifedata.com/resource/pubmed/commentcorrection/9588893-3160987, http://linkedlifedata.com/resource/pubmed/commentcorrection/9588893-3570467, http://linkedlifedata.com/resource/pubmed/commentcorrection/9588893-4018278, http://linkedlifedata.com/resource/pubmed/commentcorrection/9588893-4918317, http://linkedlifedata.com/resource/pubmed/commentcorrection/9588893-518835, http://linkedlifedata.com/resource/pubmed/commentcorrection/9588893-6195450, http://linkedlifedata.com/resource/pubmed/commentcorrection/9588893-6344641, http://linkedlifedata.com/resource/pubmed/commentcorrection/9588893-6735465, http://linkedlifedata.com/resource/pubmed/commentcorrection/9588893-72503, http://linkedlifedata.com/resource/pubmed/commentcorrection/9588893-7439972, http://linkedlifedata.com/resource/pubmed/commentcorrection/9588893-7604877, http://linkedlifedata.com/resource/pubmed/commentcorrection/9588893-7628632, http://linkedlifedata.com/resource/pubmed/commentcorrection/9588893-7886453, http://linkedlifedata.com/resource/pubmed/commentcorrection/9588893-7960119, http://linkedlifedata.com/resource/pubmed/commentcorrection/9588893-8267767, http://linkedlifedata.com/resource/pubmed/commentcorrection/9588893-8417977, http://linkedlifedata.com/resource/pubmed/commentcorrection/9588893-8454635, http://linkedlifedata.com/resource/pubmed/commentcorrection/9588893-8476558, http://linkedlifedata.com/resource/pubmed/commentcorrection/9588893-8506305, http://linkedlifedata.com/resource/pubmed/commentcorrection/9588893-8512704, http://linkedlifedata.com/resource/pubmed/commentcorrection/9588893-8613361, http://linkedlifedata.com/resource/pubmed/commentcorrection/9588893-8641770, http://linkedlifedata.com/resource/pubmed/commentcorrection/9588893-8836041, http://linkedlifedata.com/resource/pubmed/commentcorrection/9588893-8915011, http://linkedlifedata.com/resource/pubmed/commentcorrection/9588893-9038306, http://linkedlifedata.com/resource/pubmed/commentcorrection/9588893-9038346, http://linkedlifedata.com/resource/pubmed/commentcorrection/9588893-9060838, http://linkedlifedata.com/resource/pubmed/commentcorrection/9588893-9065200, http://linkedlifedata.com/resource/pubmed/commentcorrection/9588893-9169779, http://linkedlifedata.com/resource/pubmed/commentcorrection/9588893-9169780, http://linkedlifedata.com/resource/pubmed/commentcorrection/9588893-9202117, http://linkedlifedata.com/resource/pubmed/commentcorrection/9588893-9238038, http://linkedlifedata.com/resource/pubmed/commentcorrection/9588893-9268634
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
May
pubmed:issn
0002-9440
pubmed:author
pubmed:issnType
Print
pubmed:volume
152
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1247-58
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed-meshheading:9588893-Fallopian Tubes, pubmed-meshheading:9588893-Humans, pubmed-meshheading:9588893-Menstrual Cycle, pubmed-meshheading:9588893-Endometrium, pubmed-meshheading:9588893-Cervix Uteri, pubmed-meshheading:9588893-Anti-Infective Agents, pubmed-meshheading:9588893-Blood Proteins, pubmed-meshheading:9588893-Epithelium, pubmed-meshheading:9588893-Female, pubmed-meshheading:9588893-Adult, pubmed-meshheading:9588893-Middle Aged, pubmed-meshheading:9588893-RNA, Messenger, pubmed-meshheading:9588893-Immunohistochemistry, pubmed-meshheading:9588893-Gene Expression, pubmed-meshheading:9588893-DNA Primers, pubmed-meshheading:9588893-Blotting, Western, pubmed-meshheading:9588893-Defensins, pubmed-meshheading:9588893-Polymerase Chain Reaction, pubmed-meshheading:9588893-Microscopy, Confocal
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