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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
1998-6-3
|
| pubmed:abstractText |
Mechanical strain reportedly stimulates the synthesis of collagen in vascular smooth muscle cells (SMCs). The present study was designed to investigate a possible involvement of angiotensin II (Ang II) and transforming growth factor (TGF)-beta in stretch-induced collagen synthesis of cultured SMCs derived from the rabbit aortic media. SMCs were cyclically stretched at a rate of 10% elongation and 30 cycles/min for 24 h using the Flexercell strain unit (Flexcell International Corp., McKeesport, Pa.). A two-fold increase in collagen synthesis and a concurrent increase in total protein synthesis were noted in stretched SMCs. Concentration of immunoreactive Ang II in the conditioned medium was elevated under the mechanical strain. Stretch-induced collagen and total protein synthesis were inhibited by either a selective antagonist to Ang II (saralasin), an angiotensin I-converting enzyme inhibitor (captopril) or an antisense oligonucleotide for angiotensinogen mRNA. An elevated secretion of TGF-beta, both active and latent forms, was found in the medium of stretched SMCs. Saralasin inhibited the stretch-induced secretion of TGF-beta from SMCs. Stretch-induced collagen and total protein synthesis was further inhibited by either an anti-TGF-beta1 neutralizing antibody or an adenovirus-mediated transfer of a truncated TGF-beta type II receptor. Elevated expression of collagen alpha1(III) chain and TGF-beta1 mRNAs, and its reversal by saralasin were also demonstrated in stretched SMCs. Results indicate that the stretch-induced collagen and total protein synthesis appears to be mediated via an autocrine-paracrine mechanism of Ang II and TGF-beta released from SMCs.
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| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Angiotensin II,
http://linkedlifedata.com/resource/pubmed/chemical/Angiotensin-Converting Enzyme...,
http://linkedlifedata.com/resource/pubmed/chemical/Angiotensinogen,
http://linkedlifedata.com/resource/pubmed/chemical/Collagen,
http://linkedlifedata.com/resource/pubmed/chemical/Muscle Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Transforming Growth...,
http://linkedlifedata.com/resource/pubmed/chemical/Transforming Growth Factor beta
|
| pubmed:status |
MEDLINE
|
| pubmed:issn |
1018-1172
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
35
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
93-103
|
| pubmed:dateRevised |
2004-11-17
|
| pubmed:meshHeading |
pubmed-meshheading:9588872-Angiotensin II,
pubmed-meshheading:9588872-Angiotensin-Converting Enzyme Inhibitors,
pubmed-meshheading:9588872-Angiotensinogen,
pubmed-meshheading:9588872-Animals,
pubmed-meshheading:9588872-Aorta,
pubmed-meshheading:9588872-Cells, Cultured,
pubmed-meshheading:9588872-Collagen,
pubmed-meshheading:9588872-Fluorometry,
pubmed-meshheading:9588872-Male,
pubmed-meshheading:9588872-Muscle, Smooth, Vascular,
pubmed-meshheading:9588872-Muscle Proteins,
pubmed-meshheading:9588872-Protein Biosynthesis,
pubmed-meshheading:9588872-RNA, Messenger,
pubmed-meshheading:9588872-Rabbits,
pubmed-meshheading:9588872-Receptors, Transforming Growth Factor beta,
pubmed-meshheading:9588872-Stress, Mechanical,
pubmed-meshheading:9588872-Transforming Growth Factor beta,
pubmed-meshheading:9588872-Tunica Media,
pubmed-meshheading:9588872-Vasodilation
|
| pubmed:articleTitle |
Stretch-induced collagen synthesis in cultured smooth muscle cells from rabbit aortic media and a possible involvement of angiotensin II and transforming growth factor-beta.
|
| pubmed:affiliation |
First Department of Pathology, Wakayama Medical College, Japan.
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| pubmed:publicationType |
Journal Article
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