Linked Life Data

9584077

Source:http://linkedlifedata.com/resource/pubmed/id/9584077

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1998-6-16
pubmed:abstractText
Clonal populations of T cells can be identified by polymerase chain reaction (PCR) amplification of the rearranged T cell receptor gamma (TCRG) chain gene. However, because of the limited combinatorial diversity of this locus it is necessary to separate the PCR product on the basis of sequence as well as size to distinguish clonal and polyclonal T cell populations. A simple method is described which achieves this by analysing the PCR product on a single-stranded conformation polymorphism (SSCP) gel. Sensitivity has been improved by denaturing the DNA using a low ionic strength (LIS) method rather than the more conventional alkali or formamide. Results from the PCR-LIS-SSCP method on a wide range of disorders and types of tissue samples show that clonality could be demonstrated in 40/44 cases.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0890-8508
pubmed:author
pubmed:copyrightInfo
Copyright 1998 Academic Press Limited
pubmed:issnType
Print
pubmed:volume
12
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
41-8
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1998
pubmed:articleTitle
Simple, reliable detection of T cell clones by PCR-LIS-SSCP analysis of TCRgamma rearrangement.
pubmed:affiliation
Department of Haematology, Derriford Hospital, Plymouth, UK.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't