Linked Life Data

9581345

Source:http://linkedlifedata.com/resource/pubmed/id/9581345

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1998-5-29
pubmed:abstractText
The immunisation of mammals with LPS- and lipid A-antigens leads to the production of specific antibodies. In the present study, we describe the generation and preparation of antibodies from egg yolks of immunized chickens. Egg yolk antibodies were raised by immunizing laying hens with five LPS- and three lipid A-preparations from various gram-negative bacteria species in different immunisation protocols. Antibodies from collected egg yolks were extracted and purified by means of several standard methods. The purity of antibody-preparations was measured and compared by SDS-PAGE. Specific antibodies were assayed by two different EIA procedures. It could be shown that the immunisation of hens with LPS- and lipid A-Antigens resulted in the production of specific egg yolk antibodies, regardless of immunisation-scheme and amount of antigen.
pubmed:language
ger
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0005-9366
pubmed:author
pubmed:issnType
Print
pubmed:volume
111
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
121-6
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1998
pubmed:articleTitle
[Generation and characterization of avian vitelline antibodies against lipopolysaccharide and lipid A. 1. Induction and preparation of specific egg yolk antibodies (IgY) against endotoxins].
pubmed:affiliation
Institut für Virologie, Fachbereich Veterinärmedizin (Mitte), Freie Universität Berlin.
pubmed:publicationType
Journal Article, English Abstract, Research Support, Non-U.S. Gov't