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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
2
|
| pubmed:dateCreated |
1998-6-11
|
| pubmed:abstractText |
The purpose of this study was to detect the presence, and determine the normal values and the significance of DNase I in the amniotic fluid and in human placentas. Fifty-one pregnant women at 16-22 weeks of gestation and 89 women in spontaneous labour at term were recruited to the study. DNase I activity was measured in amniotic fluid and cytoplasmic extracts from the fetal portion of placentas by using a spectrophotometric technique (DNA precipitation assay) and nucleic acid electrophoresis, following degradation of the DNA by the enzyme. DNase I activity was undetectable in the placental cytoplasmic extracts. In the second trimester of pregnancy DNase I activity was detectable in amniotic fluid (2.3+/-0.64x10(5) U/l). During labour DNase I activity was also detectable, but reduced levels were observed in the presence of clear amniotic fluid, compared to second trimester levels, (1.9+/-0.44x10(5) U/l, P<0.001), whereas higher levels were found in the presence of meconium-stained amniotic fluid compared to both second trimester and clear amniotic fluid at labour (11.4+/-4.1x10(5) U/l, P<0.001 and P<0.001, respectively). Three out of 17 fetuses with meconium-stained amniotic fluid (18%) developed perinatal infection. In conclusion, the detection of DNase I activity in the amniotic fluid of second and third trimester indicates a physiological role in human pregnancy. DNase I activity normally decreases at term, compared to second trimester levels, but increases significantly in the presence of meconium.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Apr
|
| pubmed:issn |
0301-2115
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
77
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
177-80
|
| pubmed:dateRevised |
2004-11-17
|
| pubmed:meshHeading |
pubmed-meshheading:9578275-Amniotic Fluid,
pubmed-meshheading:9578275-DNA,
pubmed-meshheading:9578275-Deoxyribonuclease I,
pubmed-meshheading:9578275-Electrophoresis, Agar Gel,
pubmed-meshheading:9578275-Female,
pubmed-meshheading:9578275-Gestational Age,
pubmed-meshheading:9578275-Humans,
pubmed-meshheading:9578275-Labor, Obstetric,
pubmed-meshheading:9578275-Meconium,
pubmed-meshheading:9578275-Placenta,
pubmed-meshheading:9578275-Pregnancy,
pubmed-meshheading:9578275-Reference Values
|
| pubmed:year |
1998
|
| pubmed:articleTitle |
The role of deoxyribonuclease I in amniotic fluid during pregnancy and labour.
|
| pubmed:affiliation |
Department of Obstetrics and Gynaecology, University Hospital of Ioannina, Greece.
|
| pubmed:publicationType |
Journal Article
|