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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
9
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pubmed:dateCreated |
1998-6-1
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pubmed:abstractText |
Homologous recombination between dispersed DNA repeats creates chromosomal rearrangements that are deleterious to the genome. The methylation associated with DNA repeats in many eukaryotes might serve to inhibit homologous recombination and play a role in preserving genome integrity. We have tested the hypothesis that DNA methylation suppresses meiotic recombination in the fungus Ascobolus immersus. The natural process of methylation-induced premeiotically (MIP) was used to methylate the b2 spore color gene, a 7.5-kb chromosomal recombination hot spot. The frequency of crossing-over between two markers flanking b2 was reduced several hundredfold when b2 was methylated on the two homologs. This demonstrates that DNA methylation strongly inhibits homologous recombination. When b2 was methylated on one homolog only, crossing-over was still reduced 50-fold, indicating that the effect of methylation cannot be limited to the blocking of initiation of recombination on the methylated homolog. On the basis of these and other observations, we propose that DNA methylation perturbs pairing between the two intact homologs before recombination initiation and/or impairs the normal processing of recombination intermediates.
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pubmed:commentsCorrections |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0890-9369
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
12
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1381-9
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:9573054-Alleles,
pubmed-meshheading:9573054-Ascomycota,
pubmed-meshheading:9573054-Crossing Over, Genetic,
pubmed-meshheading:9573054-DNA, Fungal,
pubmed-meshheading:9573054-DNA Methylation,
pubmed-meshheading:9573054-Gene Rearrangement,
pubmed-meshheading:9573054-Genetic Markers,
pubmed-meshheading:9573054-Meiosis,
pubmed-meshheading:9573054-Repetitive Sequences, Nucleic Acid
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pubmed:year |
1998
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pubmed:articleTitle |
Suppression of crossing-over by DNA methylation in Ascobolus.
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pubmed:affiliation |
Institut de Génétique et Microbiologie, Université Paris-Sud, 91405 Orsay Cedex, France.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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