Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
5
|
| pubmed:dateCreated |
1998-5-18
|
| pubmed:abstractText |
In [3H]myristic acid-prelabeled Chinese hamster ovary cells stably expressing the rat NK1 tachykinin receptor, the selective NK1 agonist [Pro9]substance P ([Pro9]SP) time and concentration dependently stimulated the formation of [3H]phosphatidylethanol in the presence of ethanol. This [Pro9]SP-induced activation of phospholipase D (PLD) was blocked by NK1 receptor antagonists and poorly or not mimicked by NK2 and NK3 agonists, respectively. In confirmation of previous observations, [Pro9]SP also stimulated the hydrolysis of phosphoinositides, the release of arachidonic acid, and the formation of cyclic AMP (cAMP). All these [Pro9]SP-evoked responses could be mimicked by aluminum fluoride, but they remained unaffected in cells pretreated with pertussis toxin, suggesting that a Gi/Go protein is not involved in these different signaling pathways. The activation of PLD by [Pro9]SP was sensitive to external calcium and required an active protein kinase C because the inhibition of this kinase (Ro 31-8220) or its down-regulation (long-term treatment with a phorbol ester) abolished the response. In contrast, a cAMP-dependent process was not involved in the activation of PLD because the [Pro9]SP-evoked response was neither affected by Rp-8-bromoadenosine 3',5'-cyclic monophosphorothioate nor mimicked by cAMP-generating compounds (cholera toxin or forskolin) or by 8-bromo-cyclic AMP. A functional coupling of NK1 receptors to PLD was also demonstrated in the human astrocytoma cell line U 373 MG stimulated by SP or [Pro9]SP. These results suggest that PLD activation could be an additional signaling pathway involved in the mechanism of action of SP in target cells expressing NK1 receptors.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/Cyclic AMP,
http://linkedlifedata.com/resource/pubmed/chemical/GTP-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Glycerophospholipids,
http://linkedlifedata.com/resource/pubmed/chemical/Myristic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphatidic Acids,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphatidylcholines,
http://linkedlifedata.com/resource/pubmed/chemical/Phospholipase D,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Kinase C,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Neurokinin-1,
http://linkedlifedata.com/resource/pubmed/chemical/Substance P,
http://linkedlifedata.com/resource/pubmed/chemical/Tachykinins,
http://linkedlifedata.com/resource/pubmed/chemical/phosphatidylethanol,
http://linkedlifedata.com/resource/pubmed/chemical/substance P, Pro(9)-
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
May
|
| pubmed:issn |
0022-3042
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
70
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
2091-8
|
| pubmed:dateRevised |
2007-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:9572295-Animals,
pubmed-meshheading:9572295-Astrocytoma,
pubmed-meshheading:9572295-CHO Cells,
pubmed-meshheading:9572295-Calcium,
pubmed-meshheading:9572295-Cricetinae,
pubmed-meshheading:9572295-Cyclic AMP,
pubmed-meshheading:9572295-Enzyme Activation,
pubmed-meshheading:9572295-GTP-Binding Proteins,
pubmed-meshheading:9572295-Glycerophospholipids,
pubmed-meshheading:9572295-Humans,
pubmed-meshheading:9572295-Myristic Acid,
pubmed-meshheading:9572295-Phosphatidic Acids,
pubmed-meshheading:9572295-Phosphatidylcholines,
pubmed-meshheading:9572295-Phospholipase D,
pubmed-meshheading:9572295-Protein Kinase C,
pubmed-meshheading:9572295-Rats,
pubmed-meshheading:9572295-Receptors, Neurokinin-1,
pubmed-meshheading:9572295-Substance P,
pubmed-meshheading:9572295-Tachykinins,
pubmed-meshheading:9572295-Tumor Cells, Cultured
|
| pubmed:year |
1998
|
| pubmed:articleTitle |
Functional coupling of the NK1 tachykinin receptor to phospholipase D in chinese hamster ovary cells and astrocytoma cells.
|
| pubmed:affiliation |
Neuropharmacologie, INSERM U114, Collège de France, Paris.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|