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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1998-7-17
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pubmed:abstractText |
The acetonitrile-sonication extraction method (US EPA Method 8330) associated with aquatic-based toxicity tests was examined to study the ecotoxicity of energetic substances in soil. Three studies were carried out: (1) toxicological characterization of different energetic substances to select a representative toxicant and to validate the choice of bioassays; (2) choice of an appropriate solvent to transfer acetonitrile extracts to the bioassay incubation media; and (3) optimization of Method 8330 using soil samples spiked with the toxicant. Initial studies indicated that pure 2,4,6-trinitrotoluene (TNT) was toxic to Vibrio fischeri [Microtox; IC50 (15 min) of 4.2 microM], whereas RDX was less toxic (IC20 = 181 microM) and HMX was not toxic up to its limit of water solubility (< 22 microM). Selected pure TNT metabolites were less toxic than TNT. Similar results were found using the 96-h Selenastrum capricornutum growth inhibition test. The toxicity of pure TNT in different solvents (acetonitrile, acetone, and DMSO) and that from Method 8330-extracted TNT-spiked soil samples were compared to TNT dissolved in water. Data indicated that DMSO was the most appropriate solvent to transfer the acetonitrile extracts. A modified Method 8330 may be used in conjunction with bioassays and chemical analyses to examine the ecotoxicity of soils contaminated with energetic substances.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Acetone,
http://linkedlifedata.com/resource/pubmed/chemical/Acetonitriles,
http://linkedlifedata.com/resource/pubmed/chemical/Azocines,
http://linkedlifedata.com/resource/pubmed/chemical/Dimethyl Sulfoxide,
http://linkedlifedata.com/resource/pubmed/chemical/Heterocyclic Compounds, 1-Ring,
http://linkedlifedata.com/resource/pubmed/chemical/Rodenticides,
http://linkedlifedata.com/resource/pubmed/chemical/Soil Pollutants,
http://linkedlifedata.com/resource/pubmed/chemical/Solvents,
http://linkedlifedata.com/resource/pubmed/chemical/Triazines,
http://linkedlifedata.com/resource/pubmed/chemical/Trinitrotoluene,
http://linkedlifedata.com/resource/pubmed/chemical/acetonitrile,
http://linkedlifedata.com/resource/pubmed/chemical/cyclonite,
http://linkedlifedata.com/resource/pubmed/chemical/octogen
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pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0147-6513
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
39
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
185-94
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pubmed:dateRevised |
2010-11-18
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pubmed:meshHeading |
pubmed-meshheading:9570909-Acetone,
pubmed-meshheading:9570909-Acetonitriles,
pubmed-meshheading:9570909-Azocines,
pubmed-meshheading:9570909-Chlorophyta,
pubmed-meshheading:9570909-Chromatography, High Pressure Liquid,
pubmed-meshheading:9570909-Dimethyl Sulfoxide,
pubmed-meshheading:9570909-Dose-Response Relationship, Drug,
pubmed-meshheading:9570909-Environmental Monitoring,
pubmed-meshheading:9570909-Heterocyclic Compounds, 1-Ring,
pubmed-meshheading:9570909-Luminescent Measurements,
pubmed-meshheading:9570909-Rodenticides,
pubmed-meshheading:9570909-Soil Pollutants,
pubmed-meshheading:9570909-Solvents,
pubmed-meshheading:9570909-Toxicity Tests,
pubmed-meshheading:9570909-Triazines,
pubmed-meshheading:9570909-Trinitrotoluene,
pubmed-meshheading:9570909-Vibrio
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pubmed:year |
1998
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pubmed:articleTitle |
Development of a soil extraction procedure for ecotoxicity characterization of energetic compounds.
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pubmed:affiliation |
Biotechnology Research Institute, National Research Council of Canada, Montréal, Québec.
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pubmed:publicationType |
Journal Article,
Comparative Study
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