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rdf:type |
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lifeskim:mentions |
umls-concept:C0011306,
umls-concept:C0017262,
umls-concept:C0017337,
umls-concept:C0039195,
umls-concept:C0079189,
umls-concept:C0086418,
umls-concept:C0123759,
umls-concept:C0205225,
umls-concept:C0205349,
umls-concept:C0439859,
umls-concept:C0679058,
umls-concept:C0871261,
umls-concept:C1171362,
umls-concept:C1415900,
umls-concept:C1515670,
umls-concept:C1533691,
umls-concept:C1547699,
umls-concept:C1627358,
umls-concept:C1704632,
umls-concept:C1706817,
umls-concept:C2349975,
umls-concept:C2700640,
umls-concept:C2911692,
umls-concept:C2936596
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pubmed:issue |
3
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pubmed:dateCreated |
1998-5-14
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pubmed:abstractText |
DNA-based immunization strategies designed to elicit cellular antitumor immunity offer an attractive alternative to protein- or peptide-based approaches. In the present study we have evaluated the feasibility of DNA vaccination for the induction of CTL reactivity to five different melanoma Ags in vitro. Cultured, monocyte-derived dendritic cells (DC) were transiently transfected with plasmid DNA encoding human MART-1/Melan-A, pMel-17/gp100, tyrosinase, MAGE-1, or MAGE-3 by particle bombardment and used to stimulate autologous PBMC responder T cells. CTL reactivity to these previously identified melanoma Ags was reproducibly generated after two or three stimulations with genetically modified DC. Co-ordinate transfection of two melanoma Ag cDNAs into DC promoted CTL responders capable of recognizing epitopes from both gene products. Coinsertion of genes encoding the Th1-biasing cytokines IL-12 or IFN-alpha consistently enhanced the magnitude of the resulting Ag-specific CTL reactivity. Importantly, DC transfected with a single melanoma Ag cDNA were capable of stimulating Ag-specific CTL reactivity restricted by multiple host MHC alleles, some of which had not been previously identified. These results support the inherent strengths of gene-based vaccine approaches that do not require prior knowledge of responder MHC haplotypes or of relevant MHC-restricted peptide epitopes. Given previous observations of in situ tumor HLA allele-loss variants, DC gene vaccine strategies may elicit a greater diversity of host therapeutic immunity, thereby enhancing the clinical utility and success of such approaches.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Neoplasm,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Complementary,
http://linkedlifedata.com/resource/pubmed/chemical/Epitopes, T-Lymphocyte,
http://linkedlifedata.com/resource/pubmed/chemical/Interferon-gamma,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-12,
http://linkedlifedata.com/resource/pubmed/chemical/Luciferases,
http://linkedlifedata.com/resource/pubmed/chemical/MAGEA3 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/MART-1 Antigen,
http://linkedlifedata.com/resource/pubmed/chemical/MLANA protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Neoplasm Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Peptides,
http://linkedlifedata.com/resource/pubmed/chemical/SILV protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Vaccines, DNA,
http://linkedlifedata.com/resource/pubmed/chemical/gp100 Melanoma Antigen
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0022-1767
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pubmed:author |
pubmed-author:BabaDD,
pubmed-author:KasamonY LYL,
pubmed-author:LotzeM TMT,
pubmed-author:LyD PDP,
pubmed-author:MartinD MDM,
pubmed-author:RowleyLL,
pubmed-author:SlingluffC LCLJr,
pubmed-author:StorkusW JWJ,
pubmed-author:TütingTT,
pubmed-author:WagnerS NSN,
pubmed-author:WilsonC CCC,
pubmed-author:van der BruggenPP
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
160
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1139-47
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pubmed:dateRevised |
2010-11-18
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pubmed:meshHeading |
pubmed-meshheading:9570527-Antigens, Neoplasm,
pubmed-meshheading:9570527-Cell Line,
pubmed-meshheading:9570527-Cytotoxicity, Immunologic,
pubmed-meshheading:9570527-DNA, Complementary,
pubmed-meshheading:9570527-Dendritic Cells,
pubmed-meshheading:9570527-Epitopes, T-Lymphocyte,
pubmed-meshheading:9570527-Humans,
pubmed-meshheading:9570527-Immunophenotyping,
pubmed-meshheading:9570527-Interferon-gamma,
pubmed-meshheading:9570527-Interleukin-12,
pubmed-meshheading:9570527-Luciferases,
pubmed-meshheading:9570527-MART-1 Antigen,
pubmed-meshheading:9570527-Melanoma,
pubmed-meshheading:9570527-Membrane Glycoproteins,
pubmed-meshheading:9570527-Monocytes,
pubmed-meshheading:9570527-Mutagenesis, Insertional,
pubmed-meshheading:9570527-Neoplasm Proteins,
pubmed-meshheading:9570527-Peptides,
pubmed-meshheading:9570527-T-Lymphocytes, Cytotoxic,
pubmed-meshheading:9570527-Th1 Cells,
pubmed-meshheading:9570527-Transfection,
pubmed-meshheading:9570527-Tumor Cells, Cultured,
pubmed-meshheading:9570527-Vaccines, DNA,
pubmed-meshheading:9570527-gp100 Melanoma Antigen
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pubmed:year |
1998
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pubmed:articleTitle |
Autologous human monocyte-derived dendritic cells genetically modified to express melanoma antigens elicit primary cytotoxic T cell responses in vitro: enhancement by cotransfection of genes encoding the Th1-biasing cytokines IL-12 and IFN-alpha.
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pubmed:affiliation |
Department of Surgery, University of Pittsburgh School of Medicine, University of Pittsburgh Cancer Institute, PA 15261, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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