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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
17
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pubmed:dateCreated |
1998-5-22
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pubmed:abstractText |
Transcription factor C/EBPbeta has been known to regulate a wide array of genes including those involved in the acute-phase response. One of the molecular mechanisms underlying transcription activation by C/EBPbeta is through protein-protein interaction with other transcription factors. Here we report the identification and characterization of physical and functional interactions between C/EBPbeta and heterogeneous nuclear ribonucleoprotein (hnRNP) K. This interaction results in the repression of C/EBPbeta-dependent trans-activation of the agp gene. Footprinting assays indicate that hnRNP K cannot bind to the promoter region of agp gene or interfere with the binding of C/EBPbeta to its cognate DNA site. Furthermore, agp gene activation by the synergistic interaction of Nopp140 and C/EBPbeta is abolished by hnRNP K. The kinetics of appearance of C/EBPbeta-hnRNP K complex in the nuclear extract after initiation of acute-phase reaction indicates that hnRNP K functions as a negative regulator of C/EBPbeta-mediated activation of agp gene.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0021-9258
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
24
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pubmed:volume |
273
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
10784-91
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:9553145-Amino Acid Sequence,
pubmed-meshheading:9553145-Animals,
pubmed-meshheading:9553145-CCAAT-Enhancer-Binding Proteins,
pubmed-meshheading:9553145-Cell Line,
pubmed-meshheading:9553145-Chromatography, Affinity,
pubmed-meshheading:9553145-Cricetinae,
pubmed-meshheading:9553145-DNA-Binding Proteins,
pubmed-meshheading:9553145-Gene Expression Regulation,
pubmed-meshheading:9553145-Heterogeneous-Nuclear Ribonucleoprotein K,
pubmed-meshheading:9553145-Heterogeneous-Nuclear Ribonucleoproteins,
pubmed-meshheading:9553145-Kinetics,
pubmed-meshheading:9553145-Molecular Sequence Data,
pubmed-meshheading:9553145-Nuclear Proteins,
pubmed-meshheading:9553145-Rats,
pubmed-meshheading:9553145-Repressor Proteins,
pubmed-meshheading:9553145-Ribonucleoproteins,
pubmed-meshheading:9553145-Transcriptional Activation
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pubmed:year |
1998
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pubmed:articleTitle |
Identification of heterogeneous nuclear ribonucleoprotein K (hnRNP K) as a repressor of C/EBPbeta-mediated gene activation.
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pubmed:affiliation |
Institute of Molecular Medicine, Dr. H.-L. Tsai Memorial Laboratory, College of Medicine, National Taiwan University, Taipei, Taiwan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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