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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
15
|
| pubmed:dateCreated |
1998-5-14
|
| pubmed:databankReference | |
| pubmed:abstractText |
Selenium which occurs in proteins as the amino acid, selenocysteine, is essential for numerous biological processes and for human health. A prominent 75Se-labeled protein detected in human T-cells migrated as a 15-kDa band by SDS-polyacrylamide gel electrophoresis. This protein subunit was purified and subjected to tryptic digestion and peptide sequence analyses. Sequences of tryptic peptides derived from the protein corresponded to a human placental gene sequence containing an open reading frame of 162 residues and a readthrough in-frame TGA codon. Three different peptide sequences of the 15-kDa protein corresponded to a nucleotide sequence located downstream of this codon, suggesting that the T-cell 15-kDa selenoprotein contains a selenocysteine residue encoded by TGA. Post-translational processing of the N-terminal portion of the predicted gene product to give the 15-kDa protein was suggested on the basis of molecular mass, amino acid analysis, and immunoblot assays of the purified protein. The 3'-untranslated region (UTR) of the gene encoding the 15-kDa protein contained a sequence that is very similar to the canonical selenocysteine-inserting sequence element. Computer analysis of transcript map data bases indicated that this gene was located on human chromosome 1. Its coding sequence showed no homology to known protein-encoding genes. The 15-kDa protein gene was expressed as mRNA in a wide range of tissues, with increased levels in the thyroid, parathyroid, and prostate-derived cells as evidenced by searches of partial cDNA sequences in public data bases. Genes corresponding to the 15-kDa selenocysteine-containing protein were found in mice and rats, while the corresponding genes in Caenorhabditis elegans and Brugia malayi contained a cysteine codon in place of TGA. The discovery of a new human selenoprotein provides an additional example of the role of selenium in mammalian systems.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Complementary,
http://linkedlifedata.com/resource/pubmed/chemical/Peptide Fragments,
http://linkedlifedata.com/resource/pubmed/chemical/Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Selenium,
http://linkedlifedata.com/resource/pubmed/chemical/Selenium Radioisotopes,
http://linkedlifedata.com/resource/pubmed/chemical/Selenoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Sep15 protein, rat
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Apr
|
| pubmed:issn |
0021-9258
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
10
|
| pubmed:volume |
273
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
8910-5
|
| pubmed:dateRevised |
2005-11-17
|
| pubmed:meshHeading |
pubmed-meshheading:9535873-Amino Acid Sequence,
pubmed-meshheading:9535873-Animals,
pubmed-meshheading:9535873-Base Sequence,
pubmed-meshheading:9535873-Brugia malayi,
pubmed-meshheading:9535873-Caenorhabditis elegans,
pubmed-meshheading:9535873-Cell Line,
pubmed-meshheading:9535873-Chromosome Mapping,
pubmed-meshheading:9535873-Chromosomes, Human, Pair 1,
pubmed-meshheading:9535873-DNA, Complementary,
pubmed-meshheading:9535873-Gene Library,
pubmed-meshheading:9535873-Humans,
pubmed-meshheading:9535873-Jurkat Cells,
pubmed-meshheading:9535873-Male,
pubmed-meshheading:9535873-Mice,
pubmed-meshheading:9535873-Models, Molecular,
pubmed-meshheading:9535873-Molecular Sequence Data,
pubmed-meshheading:9535873-Molecular Weight,
pubmed-meshheading:9535873-Neoplasms,
pubmed-meshheading:9535873-Organ Specificity,
pubmed-meshheading:9535873-Peptide Fragments,
pubmed-meshheading:9535873-Peptide Mapping,
pubmed-meshheading:9535873-Protein Biosynthesis,
pubmed-meshheading:9535873-Protein Structure, Secondary,
pubmed-meshheading:9535873-Proteins,
pubmed-meshheading:9535873-Rats,
pubmed-meshheading:9535873-Recombinant Proteins,
pubmed-meshheading:9535873-Selenium,
pubmed-meshheading:9535873-Selenium Radioisotopes,
pubmed-meshheading:9535873-Selenoproteins,
pubmed-meshheading:9535873-Sequence Alignment,
pubmed-meshheading:9535873-Sequence Homology, Amino Acid,
pubmed-meshheading:9535873-T-Lymphocytes,
pubmed-meshheading:9535873-Transcription, Genetic
|
| pubmed:year |
1998
|
| pubmed:articleTitle |
A new human selenium-containing protein. Purification, characterization, and cDNA sequence.
|
| pubmed:affiliation |
Section on the Molecular Biology of Selenium, Basic Research Laboratory, NCI, National Institutes of Health, Bethesda, Maryland 20892, USA.VNG@unlinfo2.unl.edu
|
| pubmed:publicationType |
Journal Article
|