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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
15
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pubmed:dateCreated |
1998-5-14
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pubmed:abstractText |
We previously reported that interleukin-1 (IL-1) promoted the survival of murine osteoclast-like cells (OCLs) formed in vitro and activated a transcription factor, NF-kappaB, of OCLs. The present study examined whether the activation of NF-kappaB is directly involved in the survival of OCLs promoted by IL-1. The expression of IL-1 type I receptor mRNA in OCLs was detected by the polymerase chain reaction amplification of reverse-transcribed mRNA. An electrophoretic mobility shift assay showed that IL-1 transiently activated NF-kappaB in the nuclei of the OCLs, and the maximal activation occurred at 30 min. The degradation of IkappaBalpha coincided with the activation of NF-kappaB in the OCLs. The immunocytochemical study revealed that p65, a subunit of NF-kappaB, was translocated from the cytoplasm into almost all of the nuclei of the OCLs within 30 min after IL-1 stimulation. The purified OCLs spontaneously died via apoptosis, and IL-1 promoted the survival of OCLs by preventing their apoptosis. The pretreatment of purified OCLs with proteasome inhibitors suppressed the IL-1-induced activation of NF-kappaB and prevented the survival of OCLs supported by IL-1. When OCLs were pretreated with antisense oligodeoxynucleotides to p65 and p50 of NF-kappaB, the expression of respective mRNAs by OCLs was suppressed, and the IL-1-induced survival of OCLs was concomitantly inhibited. These results indicate that IL-1 promotes the survival of osteoclasts through the activation of NF-kappaB.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Cysteine Endopeptidases,
http://linkedlifedata.com/resource/pubmed/chemical/Cysteine Proteinase Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/DNA Primers,
http://linkedlifedata.com/resource/pubmed/chemical/Dactinomycin,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-1,
http://linkedlifedata.com/resource/pubmed/chemical/Multienzyme Complexes,
http://linkedlifedata.com/resource/pubmed/chemical/NF-kappa B,
http://linkedlifedata.com/resource/pubmed/chemical/Oligodeoxyribonucleotides,
http://linkedlifedata.com/resource/pubmed/chemical/Oligonucleotides, Antisense,
http://linkedlifedata.com/resource/pubmed/chemical/Proteasome Endopeptidase Complex,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Interleukin-1,
http://linkedlifedata.com/resource/pubmed/chemical/Thionucleotides
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0021-9258
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
10
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pubmed:volume |
273
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
8799-805
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:9535858-Animals,
pubmed-meshheading:9535858-Animals, Newborn,
pubmed-meshheading:9535858-Apoptosis,
pubmed-meshheading:9535858-Bone Marrow Cells,
pubmed-meshheading:9535858-Cell Survival,
pubmed-meshheading:9535858-Cysteine Endopeptidases,
pubmed-meshheading:9535858-Cysteine Proteinase Inhibitors,
pubmed-meshheading:9535858-DNA Primers,
pubmed-meshheading:9535858-Dactinomycin,
pubmed-meshheading:9535858-Interleukin-1,
pubmed-meshheading:9535858-Mice,
pubmed-meshheading:9535858-Multienzyme Complexes,
pubmed-meshheading:9535858-NF-kappa B,
pubmed-meshheading:9535858-Oligodeoxyribonucleotides,
pubmed-meshheading:9535858-Oligonucleotides, Antisense,
pubmed-meshheading:9535858-Osteoblasts,
pubmed-meshheading:9535858-Osteoclasts,
pubmed-meshheading:9535858-Polymerase Chain Reaction,
pubmed-meshheading:9535858-Proteasome Endopeptidase Complex,
pubmed-meshheading:9535858-RNA, Messenger,
pubmed-meshheading:9535858-Receptors, Interleukin-1,
pubmed-meshheading:9535858-Thionucleotides,
pubmed-meshheading:9535858-Transcription, Genetic
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pubmed:year |
1998
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pubmed:articleTitle |
Activation of NF-kappaB is involved in the survival of osteoclasts promoted by interleukin-1.
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pubmed:affiliation |
Department of Biochemistry, School of Dentistry, Showa University, 1-5-8 Hatanodai, Shinagawa-ku, Tokyo 142-8555 Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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