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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1998-5-14
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pubmed:abstractText |
Recombinant Schistosoma mansoni cyclophilin proteins of the A and the B subtypes (SmCYP A and B) were expressed in bacterial cells as histidine- and maltose-binding fusion proteins and also as nonfused proteins. In addition, S. mansoni CYPs were produced in Sf9 insect cells in their natural forms. Purified recombinant SmCYP B was found to possess a peptidyl-prolyl cis-trans isomerase (PPIase) activity, with a kcat/Km value of 8.2 x 10(5) M-1 s-1. The SmCYP B isoform is approximately two to three times more active than SmCYP A. SmCYP B-specific RNA appears to be more abundant in adult schistosomes than SmCYP A RNA in Northern blots. These results support the conclusion that SmCYP B represents the major schistosomal CYP. The PPIase-associated activity of both CYPs was inhibitable by the immunosuppressive drug cyclosporin A (CsA). We attempt to explain differences in PPIase activities and in CsA inhibition by examining models of the two CYPs complexed to CsA.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
1046-5928
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pubmed:author |
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pubmed:copyrightInfo |
Copyright 1998 Academic Press.
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pubmed:issnType |
Print
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pubmed:volume |
12
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
340-6
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:9535701-Animals,
pubmed-meshheading:9535701-Binding Sites,
pubmed-meshheading:9535701-Blotting, Western,
pubmed-meshheading:9535701-Cell Line,
pubmed-meshheading:9535701-Cloning, Molecular,
pubmed-meshheading:9535701-Cyclosporine,
pubmed-meshheading:9535701-Enzyme Inhibitors,
pubmed-meshheading:9535701-Histidine,
pubmed-meshheading:9535701-Immune Sera,
pubmed-meshheading:9535701-Maltose,
pubmed-meshheading:9535701-Models, Molecular,
pubmed-meshheading:9535701-Peptidylprolyl Isomerase,
pubmed-meshheading:9535701-Protein Conformation,
pubmed-meshheading:9535701-Rabbits,
pubmed-meshheading:9535701-Recombinant Fusion Proteins,
pubmed-meshheading:9535701-Schistosoma mansoni
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pubmed:year |
1998
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pubmed:articleTitle |
Expression cloning and biochemical characterizations of recombinant cyclophilin proteins from Schistosoma mansoni.
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pubmed:affiliation |
Istituto di Biologia Cellulare, Consiglio Nazionale delle Ricerche, 43 Viale Marx, Rome, 00137, Italy.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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