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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
1998-4-21
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pubmed:abstractText |
A monoclonal antibody (694/98-D) directed toward the V3 loop of human immunodeficiency virus type 1 (HIV-1) was evaluated for pre- and postexposure prophylaxis in SCID mice reconstituted with human peripheral blood lymphocytes (Hu-PBL-SCID). Fifty percent protection against the HIV-1LAI strain was obtained by preexposure administration of 1.32 mg/kg antibody. However, virus isolated from 1 mouse 3 weeks after passive immunization with 13.2 mg/kg antibody proved resistant to subsequent in vitro neutralization by 694/98-D. V3 loop sequence analysis of cloned virus revealed amino acid changes within the linear core epitope recognized by 694/98-D and in one flanking amino acid. Further evaluation of 694/98-D for postexposure prophylaxis in mice revealed that 694/ 98-D was effective when given 15 min after virus. However, efficacy declined to 50% if treatment was delayed to 1 h after virus inoculation. These studies point out some potential drawbacks of passive immunization with monoclonal antibodies.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0022-1899
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
177
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
889-97
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:9534960-Amino Acid Sequence,
pubmed-meshheading:9534960-Animals,
pubmed-meshheading:9534960-Antibodies, Monoclonal,
pubmed-meshheading:9534960-Cell Transplantation,
pubmed-meshheading:9534960-DNA, Viral,
pubmed-meshheading:9534960-Epitopes,
pubmed-meshheading:9534960-HIV Antibodies,
pubmed-meshheading:9534960-HIV Antigens,
pubmed-meshheading:9534960-HIV Envelope Protein gp120,
pubmed-meshheading:9534960-HIV Infections,
pubmed-meshheading:9534960-HIV-1,
pubmed-meshheading:9534960-Immunization, Passive,
pubmed-meshheading:9534960-Mice,
pubmed-meshheading:9534960-Mice, SCID,
pubmed-meshheading:9534960-Molecular Sequence Data,
pubmed-meshheading:9534960-Neutralization Tests,
pubmed-meshheading:9534960-Peptide Fragments,
pubmed-meshheading:9534960-Polymerase Chain Reaction,
pubmed-meshheading:9534960-Sequence Analysis,
pubmed-meshheading:9534960-T-Lymphocytes
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pubmed:year |
1998
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pubmed:articleTitle |
Passive immunization with a human immunodeficiency virus type 1-neutralizing monoclonal antibody in Hu-PBL-SCID mice: isolation of a neutralization escape variant.
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pubmed:affiliation |
Lindsley F. Kimball Research Institute of the New York Blood Center, New York University Medical Center, New York 10021, USA. landrus@NYBC.org
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
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