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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
|
| pubmed:dateCreated |
1998-4-28
|
| pubmed:abstractText |
mRNA expression of healthy and malignant cells can be compared to each other by employing the "differential display" (DD) technique. Most studies describe sequence analysis of differentially expressed fragments after reamplification by a second round of PCR and subsequent molecular cloning to gain a sufficient amount of DNA for sequencing. The aim of this study was to show whether a sufficient amount of differentially expressed mRNA of squamous cell carcinoma cells of the head and neck region can be generated by PCR alone without cloning steps.
|
| pubmed:language |
ger
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jan
|
| pubmed:issn |
0935-8943
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
77
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
43-7
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:9522312-Base Sequence,
pubmed-meshheading:9522312-Carcinoma, Squamous Cell,
pubmed-meshheading:9522312-Cloning, Molecular,
pubmed-meshheading:9522312-DNA, Complementary,
pubmed-meshheading:9522312-Gene Amplification,
pubmed-meshheading:9522312-Gene Expression Regulation, Neoplastic,
pubmed-meshheading:9522312-Head and Neck Neoplasms,
pubmed-meshheading:9522312-Humans,
pubmed-meshheading:9522312-Molecular Sequence Data,
pubmed-meshheading:9522312-Polymerase Chain Reaction,
pubmed-meshheading:9522312-RNA, Messenger,
pubmed-meshheading:9522312-RNA, Neoplasm
|
| pubmed:year |
1998
|
| pubmed:articleTitle |
[Re-amplification of differentially expressed mRNA fragments of head-neck cancers without cloning].
|
| pubmed:affiliation |
Klinik für Hals-, Nasen-, und Ohrenheilkunde, Kopf-und Halschirurgie, Christian-Albrechts-Universität Kiel.
|
| pubmed:publicationType |
Journal Article,
English Abstract
|