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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
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pubmed:dateCreated |
1998-5-7
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pubmed:databankReference | |
pubmed:abstractText |
The mcp gene encoding the major capsid protein (Mcp) of vibriophage KVP40, a large-tailed DNA phage, was cloned and sequenced. The nucleotide sequence of the mcp gene was 64.4% similar to that of gene 23 of coliphage T4. Analysis of the N-terminal amino acid sequence of purified native Mcp revealed that the mcp gene actually coded for a precursor, pro-Mcp, whose 62 N-terminal amino acids must be removed upon maturation to Mcp. Thus, mature Mcp would consist of 452 amino acid residues and have a calculated molecular mass of 47,561 Da. Comparison of amino acid sequences of Mcp and gp23*, the major capsid protein of T4, demonstrated 61.8% identity and 89.7% similarity between them. In addition, a sequence, TATAAATA, identical to a typical T4 late promoter sequence was seen in the region upstream of the mcp gene. These findings, together with their morphological similarity, suggest that KVP40 and T4 are phylogenetically related.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
0042-6822
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
242
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
314-8
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pubmed:dateRevised |
2004-7-12
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pubmed:meshHeading |
pubmed-meshheading:9514973-Amino Acid Sequence,
pubmed-meshheading:9514973-Bacteriophages,
pubmed-meshheading:9514973-Base Sequence,
pubmed-meshheading:9514973-Capsid,
pubmed-meshheading:9514973-Capsid Proteins,
pubmed-meshheading:9514973-Cloning, Molecular,
pubmed-meshheading:9514973-DNA, Viral,
pubmed-meshheading:9514973-Immunoblotting,
pubmed-meshheading:9514973-Immunohistochemistry,
pubmed-meshheading:9514973-Microscopy, Immunoelectron,
pubmed-meshheading:9514973-Molecular Sequence Data,
pubmed-meshheading:9514973-Sequence Homology, Nucleic Acid,
pubmed-meshheading:9514973-Vibrio,
pubmed-meshheading:9514973-Viral Proteins
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pubmed:year |
1998
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pubmed:articleTitle |
A vibriophage, KVP40, with major capsid protein homologous to gp23* of coliphage T4.
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pubmed:affiliation |
Department of Microbiology, Kochi Medical School, Japan. matuzaki@kochi-ms.ac.jp
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pubmed:publicationType |
Journal Article
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