Linked Life Data

9489891

Source:http://linkedlifedata.com/resource/pubmed/id/9489891

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1998-4-8
pubmed:abstractText
A technique for recovering functional synaptoneurosomes containing vesicularized elements of the presynapse and postsynapse into an enriched fraction has been modified to allow for small amounts of starting brain tissue. Single 400 microm rat hippocampal slices were homogenized and sequentially filtered in a 1 cc tuberculin syringe to produce an enriched synaptoneurosome fraction. Data from Western immunoblots for specific synaptic proteins suggest that these fractions are neurochemically similar to synaptosome fractions generated by sucrose gradients. Electron micrographs show that the 'small scale' preparations contain an abundant population of fused presynaptic and postsynaptic vesicularized bodies as previously published for synaptoneurosome fractions prepared from relatively large amounts of starting tissue. The single slice synaptoneurosome preparation is a quick, easy and reliable method for use in the study of synaptic function.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0165-0270
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
77
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
151-6
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1997
pubmed:articleTitle
Isolation and characterization of synaptoneurosomes from single rat hippocampal slices.
pubmed:affiliation
Department of Psychiatry and Biobehavioral Sciences, Neuropsychiatric Institute, UCLA School of Medicine, Los Angeles, CA 90024-1759, USA.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't