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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1998-3-16
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pubmed:abstractText |
The sera of patients with Lambert-Eaton myasthenic syndrome (LEMS) contain autoantibodies against several extracellular and intracellular components of the voltage-gated calcium channel (VGCC)/synaptic vesicle release complex. An example of the latter are anti-beta-subunit antibodies (anti-MysB antibodies). We constructed a full-length cDNA clone of a human VGCC beta-subunit to produce purified beta-subunit fusion protein (MysB protein). Using this protein, we demonstrated that anti-beta-subunit antibodies are present in the sera of 23% of LEMS patients and only, in low titer, in 2% of small cell lung cancer patients without LEMS. The presence of anti-beta-subunit antibodies was closely associated with high titers of P/Q- and N-type VGCC antibodies. Immunization of rats with the purified MysB protein induced high antibody titers, but no signs of neurologic dysfunction were found. We conclude that anti-beta-subunit antibodies are not likely to interfere with ion channel function, but their presence could explain the cross-reactivity of LEMS sera with several subtypes of VGCCs and the lack of correlation between anti-VGCC antibody titer and clinical severity of disease.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0028-3878
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
50
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
475-9
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:9484375-Animals,
pubmed-meshheading:9484375-Autoantibodies,
pubmed-meshheading:9484375-Blood Donors,
pubmed-meshheading:9484375-Calcium Channels,
pubmed-meshheading:9484375-Calcium Channels, L-Type,
pubmed-meshheading:9484375-Cell Membrane,
pubmed-meshheading:9484375-Cerebellum,
pubmed-meshheading:9484375-Cerebral Cortex,
pubmed-meshheading:9484375-Cloning, Molecular,
pubmed-meshheading:9484375-Electrophysiology,
pubmed-meshheading:9484375-Female,
pubmed-meshheading:9484375-Humans,
pubmed-meshheading:9484375-Lambert-Eaton Myasthenic Syndrome,
pubmed-meshheading:9484375-Macromolecular Substances,
pubmed-meshheading:9484375-Nerve Tissue Proteins,
pubmed-meshheading:9484375-Rats,
pubmed-meshheading:9484375-Rats, Inbred Lew,
pubmed-meshheading:9484375-Recombinant Fusion Proteins,
pubmed-meshheading:9484375-Reference Values
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pubmed:year |
1998
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pubmed:articleTitle |
Antibodies against the calcium channel beta-subunit in Lambert-Eaton myasthenic syndrome.
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pubmed:affiliation |
Department of Neurology, Leiden University Hospital, The Netherlands.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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