Linked Life Data

9481769

Source:http://linkedlifedata.com/resource/pubmed/id/9481769

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
1998-4-16
pubmed:databankReference
pubmed:abstractText
Infection of suckling mice with Giardia trophozoites recovered from the intestines of 11 dogs autopsied in Central and Southern Australia in each case produced an established isolate. In contrast, only 1 isolate was obtained by inoculation of faecal cysts. The organisms grew poorly in comparison with isolates from humans or non-canine animal hosts. Light microscopy revealed that the trophozoites had median bodies with the 'claw hammer' appearance typical of G. intestinalis (syn. G. duodenalis, G. lamblia) but that they differed in shape and nuclear morphology from axenic isolates of human or canine origin. Allozymic analysis of electrophoretic data representing 26 loci and phylogenetic analysis of nucleotide sequences obtained from DNA amplified from the glutamate dehydrogenase locus showed that the 11 isolates examined from Australian dogs were genetically distinct from all isolates of G. intestinalis that have been established previously from humans and animals, and also from G. muris. Both analytical methods placed 10 of the Australian canine isolates into a unique genetic lineage (designated Assemblage C) and the eleventh into a deep-rooted second branch (designated Assemblage D), each well separated from the 2 lineages (Assemblages A and B) of G. intestinalis that encompass all the genotypes known to infect humans. In contrast, 4 axenic isolates derived from dogs in Canada and Europe (the only other isolates to have been established from dogs) have genotypes characteristic of genetic Assemblages A or B. The findings indicate that the novel Giardia identified in these rural Australian dogs have a restricted host range, possibly confined to canine species. The poor success rate in establishing Giardia from dogs in vitro suggests, further, that similar genotypes may predominate as canine parasites world-wide. The absence of such organisms among isolates of Giardia that have been established from humans by propagation in suckling mice indicates that they are unlikely to infect humans. However, infection of humans by those dog-derived genotypes that grow in vitro cannot be excluded.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0031-1820
pubmed:author
pubmed:issnType
Print
pubmed:volume
116 ( Pt 1)
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
7-19
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:9481769-Animals, pubmed-meshheading:9481769-Australia, pubmed-meshheading:9481769-Base Sequence, pubmed-meshheading:9481769-DNA, Protozoan, pubmed-meshheading:9481769-Dog Diseases, pubmed-meshheading:9481769-Dogs, pubmed-meshheading:9481769-Electrophoresis, pubmed-meshheading:9481769-Genes, Protozoan, pubmed-meshheading:9481769-Giardia, pubmed-meshheading:9481769-Giardia lamblia, pubmed-meshheading:9481769-Giardiasis, pubmed-meshheading:9481769-Glutamate Dehydrogenase, pubmed-meshheading:9481769-Humans, pubmed-meshheading:9481769-Isoenzymes, pubmed-meshheading:9481769-Mice, pubmed-meshheading:9481769-Molecular Sequence Data, pubmed-meshheading:9481769-Phylogeny, pubmed-meshheading:9481769-Polymerase Chain Reaction, pubmed-meshheading:9481769-Polymorphism, Genetic, pubmed-meshheading:9481769-Sequence Alignment, pubmed-meshheading:9481769-Species Specificity
pubmed:year
1998
pubmed:articleTitle
Novel lineages of Giardia intestinalis identified by genetic analysis of organisms isolated from dogs in Australia.
pubmed:affiliation
Department of Microbiology and Immunology, University of Adelaide, Australia. pmonis@gina.adelaide.edu.au
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't