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rdf:type |
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lifeskim:mentions |
umls-concept:C0040715,
umls-concept:C0301869,
umls-concept:C0439831,
umls-concept:C0475264,
umls-concept:C0521447,
umls-concept:C0560175,
umls-concept:C0596901,
umls-concept:C0599718,
umls-concept:C0599813,
umls-concept:C0599893,
umls-concept:C0871161,
umls-concept:C1522702,
umls-concept:C1706209,
umls-concept:C1707689
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pubmed:issue |
2
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pubmed:dateCreated |
1998-3-27
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pubmed:abstractText |
Peptides containing a hydrophobic motif associated with a nuclear localization signal separated by various linkers were synthesized in solid phase. The hydrophobic sequence corresponds either to a signal peptide sequence or to a fragment of the fusion peptide of GP41 while the hydrophilic sequence is that of a nuclear localization signal. The C-termini of these peptides bear a cysteamide group that was linked to a fluorescent probe. This allowed the cellular localization of the probe to be determined as a function of the peptide sequences. The labeled peptides were then incubated with fibroblasts. Using N-biotinylated derivatives we confirmed by indirect immunofluorescence that the observed localizations corresponds to those of the peptides. The presence of a linker appears to play a role in the cellular localization. One of these peptides was successfully used to target fluorescent oligodeoxynucleotides into living cells demonstrating improved cell delivery of peptide-oligodeoxynucleotide conjugates.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0006-291X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
13
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pubmed:volume |
243
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
601-8
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:9480855-Amino Acid Sequence,
pubmed-meshheading:9480855-Biological Transport,
pubmed-meshheading:9480855-Biotinylation,
pubmed-meshheading:9480855-Calcium Channels,
pubmed-meshheading:9480855-Endocytosis,
pubmed-meshheading:9480855-Fibroblasts,
pubmed-meshheading:9480855-Fluorescent Antibody Technique,
pubmed-meshheading:9480855-Fluorescent Dyes,
pubmed-meshheading:9480855-HIV Envelope Protein gp41,
pubmed-meshheading:9480855-Humans,
pubmed-meshheading:9480855-Microscopy, Fluorescence,
pubmed-meshheading:9480855-Molecular Sequence Data,
pubmed-meshheading:9480855-Nuclear Localization Signals,
pubmed-meshheading:9480855-Oligodeoxyribonucleotides,
pubmed-meshheading:9480855-Oligonucleotides, Antisense,
pubmed-meshheading:9480855-Patch-Clamp Techniques,
pubmed-meshheading:9480855-Protein Sorting Signals
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pubmed:year |
1998
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pubmed:articleTitle |
Design of carrier peptide-oligonucleotide conjugates with rapid membrane translocation and nuclear localization properties.
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pubmed:affiliation |
Centre de Recherche de Biochimie Macromoléculaire, ERS 155 1919, Montpellier, France.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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