Linked Life Data

9480160

Source:http://linkedlifedata.com/resource/pubmed/id/9480160

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
1998-2-25
pubmed:abstractText
The preparation of platelet concentrates (PC) from pooled buffy coats has raised concern as to whether activation of lymphocytes may take place during the period of storage. Lymphocytes and HLA-DR-positive cells are of particular relevance, as they may induce a mixed lymphocyte reaction (MLR). Therefore we investigated the degree of leukocyte contamination and determined the quantity of proliferating leukocytes using the monoclonal antibody KI-67. The content of total leukocytes was (0.07 +/- 0.05) x 10(9). Immunostaining of KI-67-positive cells proved that, directly after preparation, only single leukocytes were activated. On days 1, 3 and 5 after preparation the results were identical. Isolated cells were found to be KI-67-positive but there was no significant increase in proliferating cells as would have been expected in an MLR. We conclude that there is no allogenic activation of lymphocytes taking place in these PC during storage. Therefore transfusion of activated lymphocytes seems to be extremely unlikely.
pubmed:language
ger
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
1023-2028
pubmed:author
pubmed:issnType
Print
pubmed:volume
32
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
66-8
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1994
pubmed:articleTitle
[Lymphocyte interactions in platelet concentrates from buffy coats. Determination of proliferating cells using Ki-67 antibodies].
pubmed:affiliation
Institute für Immunologie und Transfusionsmedizin, Medizinische Universität zu Lübeck, Deutschland.
pubmed:publicationType
Journal Article, English Abstract