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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
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pubmed:dateCreated |
1998-3-17
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pubmed:abstractText |
Centromere autoantibodies are commonly found in the serum of patients with some systemic autoimmune diseases. Previous studies have shown that a major human centromere autoantigen is the histone H3-like protein CENP-A. Although the human cDNA has been cloned, native CENP-A has been neither isolated nor expressed in Escherichia coli, and specific antibodies to this chromatin-associated centromere protein are not available yet. In this report, a highly charged peptide on CENP-A (residues 3-17) was used to generate a monospecific antibody that reacts by immunoblots with the 17 kDa centromeric protein. Immunofluorescence analysis showed reactivity of this anti-CENP-A serum in several but not all mammalian culture cells analyzed, suggesting that the sequence of this histone-like centromere protein could be more variable throughout evolution than originally thought. Selective extractions of human placenta nuclear proteins and immunoblot analysis indicated that CENP-A behaves in a similar way to the core histone polypeptides after nuclease digestion of chromatin. Also, immunoblot analysis demonstrated that the CENP-A peptide used as immunogen is a target region on the CENP-A molecule in several but not all CREST patients analyzed with high titers of autoantibodies to the centromere. Lastly, we found that in Jurkat cells induced to apoptosis, CENP-A remains associated with the centromere, in contrast to other human autoantigens studied during apoptosis.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies,
http://linkedlifedata.com/resource/pubmed/chemical/Autoantigens,
http://linkedlifedata.com/resource/pubmed/chemical/Chromosomal Proteins, Non-Histone,
http://linkedlifedata.com/resource/pubmed/chemical/Epitopes,
http://linkedlifedata.com/resource/pubmed/chemical/Peptide Fragments,
http://linkedlifedata.com/resource/pubmed/chemical/centromere protein A
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pubmed:status |
MEDLINE
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pubmed:month |
Jan
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pubmed:issn |
0014-5793
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
23
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pubmed:volume |
422
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
5-9
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:9475158-Amino Acid Sequence,
pubmed-meshheading:9475158-Animals,
pubmed-meshheading:9475158-Antibodies,
pubmed-meshheading:9475158-Autoantigens,
pubmed-meshheading:9475158-Autoimmune Diseases,
pubmed-meshheading:9475158-Centromere,
pubmed-meshheading:9475158-Chromosomal Proteins, Non-Histone,
pubmed-meshheading:9475158-Epitopes,
pubmed-meshheading:9475158-Fluorescent Antibody Technique,
pubmed-meshheading:9475158-HeLa Cells,
pubmed-meshheading:9475158-Humans,
pubmed-meshheading:9475158-Immunoblotting,
pubmed-meshheading:9475158-Jurkat Cells,
pubmed-meshheading:9475158-Mammals,
pubmed-meshheading:9475158-Molecular Sequence Data,
pubmed-meshheading:9475158-Peptide Fragments,
pubmed-meshheading:9475158-Placenta
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pubmed:year |
1998
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pubmed:articleTitle |
A novel centromere monospecific serum to a human autoepitope on the histone H3-like protein CENP-A.
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pubmed:affiliation |
Departamento de Bioquímica y Biología Molecular, Facultad de Ciencias, Universidad de Cádiz, Spain. manuel.valdivia@uca.es
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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