| pubmed-article:9465059 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:9465059 | lifeskim:mentions | umls-concept:C0023467 | lld:lifeskim |
| pubmed-article:9465059 | lifeskim:mentions | umls-concept:C0521447 | lld:lifeskim |
| pubmed-article:9465059 | lifeskim:mentions | umls-concept:C0040649 | lld:lifeskim |
| pubmed-article:9465059 | lifeskim:mentions | umls-concept:C1514562 | lld:lifeskim |
| pubmed-article:9465059 | lifeskim:mentions | umls-concept:C1883221 | lld:lifeskim |
| pubmed-article:9465059 | lifeskim:mentions | umls-concept:C1521761 | lld:lifeskim |
| pubmed-article:9465059 | lifeskim:mentions | umls-concept:C0599894 | lld:lifeskim |
| pubmed-article:9465059 | lifeskim:mentions | umls-concept:C0220905 | lld:lifeskim |
| pubmed-article:9465059 | lifeskim:mentions | umls-concept:C1883204 | lld:lifeskim |
| pubmed-article:9465059 | lifeskim:mentions | umls-concept:C1880389 | lld:lifeskim |
| pubmed-article:9465059 | pubmed:issue | 4 | lld:pubmed |
| pubmed-article:9465059 | pubmed:dateCreated | 1998-3-19 | lld:pubmed |
| pubmed-article:9465059 | pubmed:abstractText | The AML/CBFalpha runt transcription factors are key regulators of hematopoietic and bone tissue-specific gene expression. These factors contain a 31-amino acid nuclear matrix targeting signal that supports association with the nuclear matrix. We determined that the AML/CBFalpha factors must bind to the nuclear matrix to exert control of transcription. Fusing the nuclear matrix targeting signal to the GAL4 DNA binding domain transactivates a genomically integrated GAL4 responsive reporter gene. These data suggest that AML/CBFalpha must associate with the nuclear matrix to effect transcription. We used fluorescence labeling of epitope-tagged AML-1B (CBFA2) to show it colocalizes with a subset of hyperphosphorylated RNA polymerase II molecules concentrated in foci and linked to the nuclear matrix. This association of AML-1B with RNA polymerase II requires active transcription and a functional DNA binding domain. The nuclear matrix domains that contain AML-1B are distinct from SC35 RNA processing domains. Our results suggest two of the requirements for AML-dependent transcription initiation by RNA polymerase II are association of AML-1B with the nuclear matrix together with specific binding of AML to gene promoters. | lld:pubmed |
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| pubmed-article:9465059 | pubmed:language | eng | lld:pubmed |
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| pubmed-article:9465059 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:9465059 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:9465059 | pubmed:month | Feb | lld:pubmed |
| pubmed-article:9465059 | pubmed:issn | 0027-8424 | lld:pubmed |
| pubmed-article:9465059 | pubmed:author | pubmed-author:LiaoJ CJC | lld:pubmed |
| pubmed-article:9465059 | pubmed:author | pubmed-author:SteimJ MJM | lld:pubmed |
| pubmed-article:9465059 | pubmed:author | pubmed-author:SteinG SGS | lld:pubmed |
| pubmed-article:9465059 | pubmed:author | pubmed-author:PenmanSS | lld:pubmed |
| pubmed-article:9465059 | pubmed:author | pubmed-author:LawrenceJ BJB | lld:pubmed |
| pubmed-article:9465059 | pubmed:author | pubmed-author:McNeilSS | lld:pubmed |
| pubmed-article:9465059 | pubmed:author | pubmed-author:NickersonJJ | lld:pubmed |
| pubmed-article:9465059 | pubmed:author | pubmed-author:van WijnenA... | lld:pubmed |
| pubmed-article:9465059 | pubmed:author | pubmed-author:ZensWW | lld:pubmed |
| pubmed-article:9465059 | pubmed:author | pubmed-author:PockwinseSS | lld:pubmed |
| pubmed-article:9465059 | pubmed:author | pubmed-author:HiebertSS | lld:pubmed |
| pubmed-article:9465059 | pubmed:author | pubmed-author:ShoplandLL | lld:pubmed |
| pubmed-article:9465059 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:9465059 | pubmed:day | 17 | lld:pubmed |
| pubmed-article:9465059 | pubmed:volume | 95 | lld:pubmed |
| pubmed-article:9465059 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:9465059 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:9465059 | pubmed:pagination | 1585-9 | lld:pubmed |
| pubmed-article:9465059 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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| pubmed-article:9465059 | pubmed:year | 1998 | lld:pubmed |
| pubmed-article:9465059 | pubmed:articleTitle | Intranuclear targeting of AML/CBFalpha regulatory factors to nuclear matrix-associated transcriptional domains. | lld:pubmed |
| pubmed-article:9465059 | pubmed:affiliation | Department of Cell Biology and Cancer Center, University of Massachusetts Medical Center, 55 Lake Avenue North, Worcester, MA 01655, USA. | lld:pubmed |
| pubmed-article:9465059 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:9465059 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
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