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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
1998-3-19
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pubmed:abstractText |
The AML/CBFalpha runt transcription factors are key regulators of hematopoietic and bone tissue-specific gene expression. These factors contain a 31-amino acid nuclear matrix targeting signal that supports association with the nuclear matrix. We determined that the AML/CBFalpha factors must bind to the nuclear matrix to exert control of transcription. Fusing the nuclear matrix targeting signal to the GAL4 DNA binding domain transactivates a genomically integrated GAL4 responsive reporter gene. These data suggest that AML/CBFalpha must associate with the nuclear matrix to effect transcription. We used fluorescence labeling of epitope-tagged AML-1B (CBFA2) to show it colocalizes with a subset of hyperphosphorylated RNA polymerase II molecules concentrated in foci and linked to the nuclear matrix. This association of AML-1B with RNA polymerase II requires active transcription and a functional DNA binding domain. The nuclear matrix domains that contain AML-1B are distinct from SC35 RNA processing domains. Our results suggest two of the requirements for AML-dependent transcription initiation by RNA polymerase II are association of AML-1B with the nuclear matrix together with specific binding of AML to gene promoters.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/9465059-1423634,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9465059-6202700,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9465059-7479833,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9465059-7536746,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9465059-7548073,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9465059-7962188,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9465059-8320255,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9465059-8462102,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9465059-8557490,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9465059-8557734,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9465059-8657108,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9465059-8701093,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9465059-8710856,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9465059-8761951,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9465059-8799830,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9465059-8834230,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9465059-8834231,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9465059-8844400,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9465059-8844402,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9465059-8913879,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9465059-8947496,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9465059-8977438,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9465059-9057107,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9465059-9128241,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9465059-9182762,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9465059-9192636,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9465059-9215522,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9465059-9215534,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9465059-9264465,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9465059-9291093,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9465059-9291094
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Feb
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pubmed:issn |
0027-8424
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pubmed:author |
pubmed-author:HiebertSS,
pubmed-author:LawrenceJ BJB,
pubmed-author:LiaoJ CJC,
pubmed-author:McNeilSS,
pubmed-author:NickersonJJ,
pubmed-author:PenmanSS,
pubmed-author:PockwinseSS,
pubmed-author:ShoplandLL,
pubmed-author:SteimJ MJM,
pubmed-author:SteinG SGS,
pubmed-author:ZensWW,
pubmed-author:van WijnenA JAJ
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pubmed:issnType |
Print
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pubmed:day |
17
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pubmed:volume |
95
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1585-9
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:9465059-Cell Compartmentation,
pubmed-meshheading:9465059-Core Binding Factor Alpha 2 Subunit,
pubmed-meshheading:9465059-DNA-Binding Proteins,
pubmed-meshheading:9465059-HeLa Cells,
pubmed-meshheading:9465059-Humans,
pubmed-meshheading:9465059-Microscopy, Fluorescence,
pubmed-meshheading:9465059-Nuclear Matrix,
pubmed-meshheading:9465059-Proto-Oncogene Proteins,
pubmed-meshheading:9465059-RNA Polymerase II,
pubmed-meshheading:9465059-Transcription, Genetic,
pubmed-meshheading:9465059-Transcription Factors,
pubmed-meshheading:9465059-Transcriptional Activation,
pubmed-meshheading:9465059-Transfection
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pubmed:year |
1998
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pubmed:articleTitle |
Intranuclear targeting of AML/CBFalpha regulatory factors to nuclear matrix-associated transcriptional domains.
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pubmed:affiliation |
Department of Cell Biology and Cancer Center, University of Massachusetts Medical Center, 55 Lake Avenue North, Worcester, MA 01655, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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