Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
1998-4-9
pubmed:databankReference
pubmed:abstractText
Carnitine palmitoyltransferase I (CPTI) catalyses the transfer of long chain fatty acids to carnitine for translocation across the mitochondrial inner membrane. The cDNAs of two isoforms of CPT I, termed the hepatic and muscle isoforms, have been cloned. Expression of the hepatic CPT I gene (L-CPT I) is subject to developmental, hormonal and tissue specific regulation. We have cloned the promoter of the L-CPTI gene from a rat genomic library. In the L-CPTI gene, there are two exons 5' to the exon containing the ATG that initiates translation. Exon 1 and the 5' end of exon 2 contain sequences that were not previously described in the rat L-CPTI cDNA. There is an alternatively spliced form of the L-CPTI mRNA in which exon 2 is skipped. The proximal promoter of the L-CPTI gene is extremely GC rich and does not contain a TATA box. There are several putative Sp1 binding sites near the transcriptional start site. A 190 base pair fragment of the promoter can efficiently drive transcription of luciferase and CAT (chloramphenicol acetyltransferase) reporter genes transiently transfected into HepG2 cells. Sequences in both the first intron and the promoter contribute to basal expression. Our results provide the foundation for further studies into the regulation of L-CPTI gene expression.
pubmed:grant
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/9461513-1657708, http://linkedlifedata.com/resource/pubmed/commentcorrection/9461513-1875951, http://linkedlifedata.com/resource/pubmed/commentcorrection/9461513-2355018, http://linkedlifedata.com/resource/pubmed/commentcorrection/9461513-2590167, http://linkedlifedata.com/resource/pubmed/commentcorrection/9461513-2697636, http://linkedlifedata.com/resource/pubmed/commentcorrection/9461513-2822255, http://linkedlifedata.com/resource/pubmed/commentcorrection/9461513-3435481, http://linkedlifedata.com/resource/pubmed/commentcorrection/9461513-3656413, http://linkedlifedata.com/resource/pubmed/commentcorrection/9461513-7495866, http://linkedlifedata.com/resource/pubmed/commentcorrection/9461513-7532283, http://linkedlifedata.com/resource/pubmed/commentcorrection/9461513-7559627, http://linkedlifedata.com/resource/pubmed/commentcorrection/9461513-7575418, http://linkedlifedata.com/resource/pubmed/commentcorrection/9461513-7639710, http://linkedlifedata.com/resource/pubmed/commentcorrection/9461513-7721804, http://linkedlifedata.com/resource/pubmed/commentcorrection/9461513-7729550, http://linkedlifedata.com/resource/pubmed/commentcorrection/9461513-7842017, http://linkedlifedata.com/resource/pubmed/commentcorrection/9461513-7892212, http://linkedlifedata.com/resource/pubmed/commentcorrection/9461513-8002965, http://linkedlifedata.com/resource/pubmed/commentcorrection/9461513-8003033, http://linkedlifedata.com/resource/pubmed/commentcorrection/9461513-8034571, http://linkedlifedata.com/resource/pubmed/commentcorrection/9461513-8086471, http://linkedlifedata.com/resource/pubmed/commentcorrection/9461513-8093246, http://linkedlifedata.com/resource/pubmed/commentcorrection/9461513-8264580, http://linkedlifedata.com/resource/pubmed/commentcorrection/9461513-8307575, http://linkedlifedata.com/resource/pubmed/commentcorrection/9461513-8319595, http://linkedlifedata.com/resource/pubmed/commentcorrection/9461513-8384714, http://linkedlifedata.com/resource/pubmed/commentcorrection/9461513-8449948, http://linkedlifedata.com/resource/pubmed/commentcorrection/9461513-8521508, http://linkedlifedata.com/resource/pubmed/commentcorrection/9461513-8626491, http://linkedlifedata.com/resource/pubmed/commentcorrection/9461513-8636126, http://linkedlifedata.com/resource/pubmed/commentcorrection/9461513-8654430, http://linkedlifedata.com/resource/pubmed/commentcorrection/9461513-8662647, http://linkedlifedata.com/resource/pubmed/commentcorrection/9461513-8679700, http://linkedlifedata.com/resource/pubmed/commentcorrection/9461513-874089, http://linkedlifedata.com/resource/pubmed/commentcorrection/9461513-8874496, http://linkedlifedata.com/resource/pubmed/commentcorrection/9461513-9063439, http://linkedlifedata.com/resource/pubmed/commentcorrection/9461513-9070950, http://linkedlifedata.com/resource/pubmed/commentcorrection/9461513-9116046, http://linkedlifedata.com/resource/pubmed/commentcorrection/9461513-9154804, http://linkedlifedata.com/resource/pubmed/commentcorrection/9461513-9199240, http://linkedlifedata.com/resource/pubmed/commentcorrection/9461513-9224698, http://linkedlifedata.com/resource/pubmed/commentcorrection/9461513-9234716
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0264-6021
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
330 ( Pt 1)
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
217-24
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1998
pubmed:articleTitle
Cloning and characterization of the promoter for the liver isoform of the rat carnitine palmitoyltransferase I (L-CPT I) gene.
pubmed:affiliation
Department of Pharmacology, University of Tennessee, College of Medicine, 874 Union Avenue, Memphis, TN 38163, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't