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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1998-2-3
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pubmed:abstractText |
CBP functions as a key transcriptional coactivator for a variety of transcription factors. We show here that the hepatocyte nuclear factor 4 (HNF4), a transcription factor in the nuclear receptor superfamily with no defined ligand, is cloned by yeast two-hybrid system using CBP as a bait. GST-pull down assay with nuclear extracts or in vitro translation products revealed that CBP and HNF4 interact with each other at the middle portion (aa 119-375) of HNF4 and two distinct regions (aa 271-451 and 1626-2259) of CBP, respectively, in the ligand-independent manner. Co-transfection experiments indicated that CBP is capable of activating HNF4 site-mediated transcription. These results suggested a functional association between CBP and HNF4 in trans-activation.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Basic Helix-Loop-Helix Leucine...,
http://linkedlifedata.com/resource/pubmed/chemical/CREB-Binding Protein,
http://linkedlifedata.com/resource/pubmed/chemical/Crebbp protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Crebbp protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Hepatocyte Nuclear Factor 4,
http://linkedlifedata.com/resource/pubmed/chemical/Nuclear Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Phosphoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Tcfl4 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Trans-Activators,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0006-291X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
29
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pubmed:volume |
241
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
664-9
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:9434765-Animals,
pubmed-meshheading:9434765-Basic Helix-Loop-Helix Leucine Zipper Transcription Factors,
pubmed-meshheading:9434765-CREB-Binding Protein,
pubmed-meshheading:9434765-Cloning, Molecular,
pubmed-meshheading:9434765-DNA-Binding Proteins,
pubmed-meshheading:9434765-Hepatocyte Nuclear Factor 4,
pubmed-meshheading:9434765-Liver,
pubmed-meshheading:9434765-Mice,
pubmed-meshheading:9434765-Nuclear Proteins,
pubmed-meshheading:9434765-Phosphoproteins,
pubmed-meshheading:9434765-Rats,
pubmed-meshheading:9434765-Recombinant Fusion Proteins,
pubmed-meshheading:9434765-Saccharomyces cerevisiae,
pubmed-meshheading:9434765-Trans-Activators,
pubmed-meshheading:9434765-Transcription, Genetic,
pubmed-meshheading:9434765-Transcription Factors,
pubmed-meshheading:9434765-Transfection
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pubmed:year |
1997
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pubmed:articleTitle |
Functional association between CBP and HNF4 in trans-activation.
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pubmed:affiliation |
Institute of Applied Biochemistry, University of Tsukuba, Ibaraki, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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