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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
4
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pubmed:dateCreated |
1998-2-12
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pubmed:abstractText |
In order to detect apoptotic cells in the kidney, the terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end-labeling (TUNEL) method is used in tissue sections. But the number of apoptotic cells per glomerulus in several experimental models of nephritides is about 1 per single tissue section even at peak levels. In this study, we have reported that the TUNEL method and immunostaining of cell-specific markers to a whole isolated glomerulus in combination with laser scan microscopy are potentially useful methods for the analysis of cell turnover within glomeruli.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:issn |
0028-2766
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
77
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
474-8
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:9434072-Animals,
pubmed-meshheading:9434072-Apoptosis,
pubmed-meshheading:9434072-Cell Count,
pubmed-meshheading:9434072-DNA Fragmentation,
pubmed-meshheading:9434072-DNA Nucleotidylexotransferase,
pubmed-meshheading:9434072-Deoxyuracil Nucleotides,
pubmed-meshheading:9434072-Kidney Glomerulus,
pubmed-meshheading:9434072-Macrophages, Peritoneal,
pubmed-meshheading:9434072-Rats,
pubmed-meshheading:9434072-Rats, Wistar,
pubmed-meshheading:9434072-Renal Circulation
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pubmed:year |
1997
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pubmed:articleTitle |
Quantification of apoptotic cells using isolated glomeruli.
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pubmed:affiliation |
Department of Cell Biology, Niigata University School of Medicine, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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