Linked Life Data

9417809

Source:http://linkedlifedata.com/resource/pubmed/id/9417809

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
12
pubmed:dateCreated
1998-2-27
pubmed:abstractText
Studies of the CD25 gene promoter region in T cells have revealed the presence of a 31-bp region, including an 11-bp negative regulatory element (NRE), which profoundly suppresses CD25 expression. This report illustrates that the same region acts as a negative regulator of CD25 expression in human B lymphocytes. Human B cells contain DNA-binding protein activities which bind specifically to an oligonucleotide equivalent to the 11-bp core region of the NRE, and stimulation of tonsillar B cells or Daudi Burkitt's lymphoma B cells with interleukin 4 (IL-4) results in loss of binding activity for oligonucleotides containing the NRE; in contrast, IL-4 enhanced binding activity for the NRE in the Jurkat T cell line. Transient transfection analyses using deletion mutants lacking both the 11-bp core NRE and both the NRE and an adjacent putative retinoic acid response element (RARE) motif illustrated that the NRE element is a functional suppressor of CD25 transcription in B cells. Thus, deletion of the NRE element increased the basal level of CD25 promoter activity and also conferred IL-4 inducibility on reporter gene expression in transiently transfected tonsillar B lymphocytes.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
1043-4666
pubmed:author
pubmed:copyrightInfo
Copyright 1997 Academic Press Limited.
pubmed:issnType
Print
pubmed:volume
9
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
982-91
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:9417809-B-Lymphocytes, pubmed-meshheading:9417809-Base Sequence, pubmed-meshheading:9417809-Chloramphenicol O-Acetyltransferase, pubmed-meshheading:9417809-DNA-Binding Proteins, pubmed-meshheading:9417809-Genes, Reporter, pubmed-meshheading:9417809-Humans, pubmed-meshheading:9417809-Interleukin-4, pubmed-meshheading:9417809-Jurkat Cells, pubmed-meshheading:9417809-Palatine Tonsil, pubmed-meshheading:9417809-Polymerase Chain Reaction, pubmed-meshheading:9417809-Promoter Regions, Genetic, pubmed-meshheading:9417809-Receptors, Interleukin-2, pubmed-meshheading:9417809-Recombinant Fusion Proteins, pubmed-meshheading:9417809-Regulatory Sequences, Nucleic Acid, pubmed-meshheading:9417809-T-Lymphocytes, pubmed-meshheading:9417809-Transfection, pubmed-meshheading:9417809-Tretinoin
pubmed:year
1997
pubmed:articleTitle
Negative regulation of the interleukin (IL)-2 receptor alpha chain (CD25) promoter region in human B lymphocytes.
pubmed:affiliation
Division of Biochemistry and Molecular Biology, Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow, G12 8QQ, UK.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't