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pubmed-article:9397347pubmed:abstractTextTo further elucidate the cellular mechanisms that mediate programmed cell death in avian immune cells, differential display analysis was employed to identify differentially expressed genes in chicken thymocytes undergoing apoptosis. Primary cultures of thymocytes were treated with dexamethasone to activate apoptosis and RNA was isolated for differential display analysis. A differential display product designated A1 (479 bp) was identified. This display product was subcloned and induced expression of the genes was confirmed by ribonuclease protection analysis. Nucleotide sequence analysis of A1 revealed a putative 82 amino acid open reading frame that demonstrated limited homology with Bad, an apoptotic regulatory protein. Thus, A1 may represent the avian homolog of Bad.lld:pubmed
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pubmed-article:9397347pubmed:authorpubmed-author:ThomsonJ MJMlld:pubmed
pubmed-article:9397347pubmed:authorpubmed-author:ComptonM MMMlld:pubmed
pubmed-article:9397347pubmed:authorpubmed-author:WaldripH WHWlld:pubmed
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pubmed-article:9397347pubmed:pagination413-24lld:pubmed
pubmed-article:9397347pubmed:dateRevised2006-11-15lld:pubmed
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pubmed-article:9397347pubmed:articleTitleIdentification of a differential display product associated with apoptosis in chicken thymocytes.lld:pubmed
pubmed-article:9397347pubmed:affiliationDepartment of Poultry Science, University of Georgia, Athens 30602-2772, USA.lld:pubmed
pubmed-article:9397347pubmed:publicationTypeJournal Articlelld:pubmed
pubmed-article:9397347pubmed:publicationTypeResearch Support, U.S. Gov't, Non-P.H.S.lld:pubmed
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