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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
12
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pubmed:dateCreated |
1997-12-23
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pubmed:abstractText |
A modified platelet response to aggregating stimuli is supposed to play a role in the pathogenesis of diabetic macroangiopathy. We studied the fluidity and microheterogeneity of the external surface of the platelet membrane and the activities of the plasma membrane Na+-K+-ATPase and Ca2+-ATPase in 21 men with type 1 diabetes and in 20 control subjects before and after in vitro thrombin addition. In the resting state, platelets from type 1 diabetic patients showed an increased fluidity and microheterogeneity of the platelet membrane, a higher Ca2+-ATPase activity, and a reduced Na+-K+-ATPase activity in comparison with platelets from healthy subjects. The fatty acid composition was also modified, with increased C 16:1 and decreased C 18:0 content. Control cells incubated with thrombin showed a modification of the membrane parameters opposite to the response observed in type 1 cells after the stimulation. The incubation of control platelets in the resting state with high concentrations of glucose modified the fluidity of the plasma membrane Na+-K+-ATPase and Ca2+-ATPase activities in an opposite way in comparison with the alterations observed in type 1 platelets. This study suggests that in type 1 diabetic patients, the platelet membrane responds to activation with a molecular remodeling different from the response of healthy subjects. The abnormal organization of the membrane might contribute to the altered platelet functions in type 1 diabetic patients, but acute exposure to high glucose levels does not seem able to modify the platelet membrane in the way observed in type 1 diabetes.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
AIM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/1-(4-(trimethylamino)phenyl)-6-pheny...,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium-Transporting ATPases,
http://linkedlifedata.com/resource/pubmed/chemical/Diphenylhexatriene,
http://linkedlifedata.com/resource/pubmed/chemical/Fatty Acids,
http://linkedlifedata.com/resource/pubmed/chemical/Fluorescent Dyes,
http://linkedlifedata.com/resource/pubmed/chemical/Glucose,
http://linkedlifedata.com/resource/pubmed/chemical/Sodium-Potassium-Exchanging ATPase
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0012-1797
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
46
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2069-74
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pubmed:dateRevised |
2010-11-18
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pubmed:meshHeading |
pubmed-meshheading:9392498-Adult,
pubmed-meshheading:9392498-Blood Platelets,
pubmed-meshheading:9392498-Calcium-Transporting ATPases,
pubmed-meshheading:9392498-Cell Membrane,
pubmed-meshheading:9392498-Diabetes Mellitus, Type 1,
pubmed-meshheading:9392498-Diphenylhexatriene,
pubmed-meshheading:9392498-Fatty Acids,
pubmed-meshheading:9392498-Fluorescence Polarization,
pubmed-meshheading:9392498-Fluorescent Dyes,
pubmed-meshheading:9392498-Glucose,
pubmed-meshheading:9392498-Humans,
pubmed-meshheading:9392498-Male,
pubmed-meshheading:9392498-Membrane Fluidity,
pubmed-meshheading:9392498-Sodium-Potassium-Exchanging ATPase
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pubmed:year |
1997
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pubmed:articleTitle |
Altered platelet membrane dynamic properties in type 1 diabetes.
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pubmed:affiliation |
Institute of Biochemistry, University of Ancona, INRCA, Italy. mazzanti@popcsi.unian.it
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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