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rdf:type |
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lifeskim:mentions |
umls-concept:C0001613,
umls-concept:C0007776,
umls-concept:C0022655,
umls-concept:C0027882,
umls-concept:C0043481,
umls-concept:C0178719,
umls-concept:C0242485,
umls-concept:C0449444,
umls-concept:C1550548,
umls-concept:C1555714,
umls-concept:C1705654,
umls-concept:C1880497,
umls-concept:C1996904
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pubmed:issue |
24
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pubmed:dateCreated |
1998-1-7
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pubmed:abstractText |
We used the ratioable fluorescent dye mag-fura-5 to measure intracellular free Zn2+ ([Zn2+]i) in cultured neocortical neurons exposed to neurotoxic concentrations of Zn2+ in concert with depolarization or glutamate receptor activation and identified four routes of Zn2+ entry. Neurons exposed to extracellular Zn2+ plus high K+ responded with a peak cell body signal corresponding to a [Zn2+]i of 35-45 nM. This increase in [Zn2+]i was attenuated by concurrent addition of Gd3+, verapamil, omega-conotoxin GVIA, or nimodipine, consistent with Zn2+ entry through voltage-gated Ca2+channels. Furthermore, under conditions favoring reverse operation of the Na+-Ca2+ exchanger, Zn2+ application induced a slow increase in [Zn2+]i and outward whole-cell current sensitive to benzamil-amiloride. Thus, a second route of Zn2+ entry into neurons may be via transporter-mediated exchange with intracellular Na+. Both NMDA and kainate also induced rapid increases in neuronal [Zn2+]i. The NMDA-induced increase was only partly sensitive to Gd3+ or to removal of extracellular Na+, consistent with a third route of entry directly through NMDA receptor-gated channels. The kainate-induced increase was highly sensitive to Gd3+ or Na+ removal in most neurons but insensitive in a minority subpopulation ("cobalt-positive cells"), suggesting that a fourth route of neuronal Zn2+ entry is through the Ca2+-permeable channels gated by certain subtypes of AMPA or kainate receptors.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/2,3-dioxo-6-nitro-7-sulfamoylbenzo(f...,
http://linkedlifedata.com/resource/pubmed/chemical/4-bromo-A-23187,
http://linkedlifedata.com/resource/pubmed/chemical/Calcimycin,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium Channel Blockers,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium Channels,
http://linkedlifedata.com/resource/pubmed/chemical/Excitatory Amino Acid Agonists,
http://linkedlifedata.com/resource/pubmed/chemical/Excitatory Amino Acid Antagonists,
http://linkedlifedata.com/resource/pubmed/chemical/Fluorescent Dyes,
http://linkedlifedata.com/resource/pubmed/chemical/Fura-2,
http://linkedlifedata.com/resource/pubmed/chemical/Ionophores,
http://linkedlifedata.com/resource/pubmed/chemical/Kainic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/Magnesium,
http://linkedlifedata.com/resource/pubmed/chemical/N-Methylaspartate,
http://linkedlifedata.com/resource/pubmed/chemical/Nimodipine,
http://linkedlifedata.com/resource/pubmed/chemical/Quinoxalines,
http://linkedlifedata.com/resource/pubmed/chemical/Sodium-Calcium Exchanger,
http://linkedlifedata.com/resource/pubmed/chemical/Zinc,
http://linkedlifedata.com/resource/pubmed/chemical/alpha-Amino-3-hydroxy-5-methyl-4-iso...,
http://linkedlifedata.com/resource/pubmed/chemical/mag-Fura 5
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0270-6474
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
17
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
9554-64
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:9391010-Animals,
pubmed-meshheading:9391010-Binding, Competitive,
pubmed-meshheading:9391010-Biological Transport,
pubmed-meshheading:9391010-Calcimycin,
pubmed-meshheading:9391010-Calcium,
pubmed-meshheading:9391010-Calcium Channel Blockers,
pubmed-meshheading:9391010-Calcium Channels,
pubmed-meshheading:9391010-Cells, Cultured,
pubmed-meshheading:9391010-Electric Stimulation,
pubmed-meshheading:9391010-Electrophysiology,
pubmed-meshheading:9391010-Excitatory Amino Acid Agonists,
pubmed-meshheading:9391010-Excitatory Amino Acid Antagonists,
pubmed-meshheading:9391010-Fluorescent Dyes,
pubmed-meshheading:9391010-Fura-2,
pubmed-meshheading:9391010-Ionophores,
pubmed-meshheading:9391010-Kainic Acid,
pubmed-meshheading:9391010-Magnesium,
pubmed-meshheading:9391010-Membrane Potentials,
pubmed-meshheading:9391010-Mice,
pubmed-meshheading:9391010-N-Methylaspartate,
pubmed-meshheading:9391010-Neocortex,
pubmed-meshheading:9391010-Neurons,
pubmed-meshheading:9391010-Nimodipine,
pubmed-meshheading:9391010-Quinoxalines,
pubmed-meshheading:9391010-Sodium-Calcium Exchanger,
pubmed-meshheading:9391010-Zinc,
pubmed-meshheading:9391010-alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid
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pubmed:year |
1997
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pubmed:articleTitle |
Measurement of intracellular free zinc in living cortical neurons: routes of entry.
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pubmed:affiliation |
Center for the Study of Nervous System Injury and Department of Neurology, Washington University School of Medicine, St. Louis, Missouri 63110, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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