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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1-2
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pubmed:dateCreated |
1998-2-11
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pubmed:abstractText |
The 5' flanking region of the mouse calretinin gene was cloned and a 1.8 kbp region adjacent to exon 1 was sequenced. Putative upstream promoter and enhancer elements were identified, including appropriately positioned TATA and CAAT boxes (positions -50 and -68, respectively). There was considerable sequence and structural homology between mouse and human upstream elements. Neuron-restrictive activity was demonstrated via transfection of calretinin promoter-reporter constructs into primary embryonic mouse brain cultures expressing calretinin. In promoterless reporter constructs, the proximal upstream 1.5 kbp of the mouse calretinin gene boosted luciferase activity (up to 100-fold) exclusively in the neuronal population. Deletion analysis revealed the minimal promoter to be within the 95-bp proximal to the transcription start site. Transfections with SV40 promoter constructs in these cultures resulted in reporter gene expression predominantly in non-neuronal cells. Inserting the proximal 1.5 kbp of mouse calretinin upstream in SV40 promoter-reporter constructs reduced luciferase activity. Thus, calretinin upstream sequences increased reporter expression in cultured neurons and decreased expression from the SV40 promoter in non-neuronal cultured brain cells. The calretinin promoter contained relevant regulatory element consensus motifs and demonstrated in vitro neuron-restrictive bioactivity.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0169-328X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
3
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pubmed:volume |
49
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
175-87
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:9387877-3T3 Cells,
pubmed-meshheading:9387877-Animals,
pubmed-meshheading:9387877-Base Sequence,
pubmed-meshheading:9387877-Binding Sites,
pubmed-meshheading:9387877-Brain,
pubmed-meshheading:9387877-Calcium-Binding Protein, Vitamin D-Dependent,
pubmed-meshheading:9387877-Cells, Cultured,
pubmed-meshheading:9387877-Cloning, Molecular,
pubmed-meshheading:9387877-DNA-Binding Proteins,
pubmed-meshheading:9387877-Female,
pubmed-meshheading:9387877-Genes, Reporter,
pubmed-meshheading:9387877-Humans,
pubmed-meshheading:9387877-Luciferases,
pubmed-meshheading:9387877-Mice,
pubmed-meshheading:9387877-Molecular Sequence Data,
pubmed-meshheading:9387877-Nerve Tissue Proteins,
pubmed-meshheading:9387877-Neurons,
pubmed-meshheading:9387877-PC12 Cells,
pubmed-meshheading:9387877-Promoter Regions, Genetic,
pubmed-meshheading:9387877-Rats,
pubmed-meshheading:9387877-Recombinant Fusion Proteins,
pubmed-meshheading:9387877-Regulatory Sequences, Nucleic Acid,
pubmed-meshheading:9387877-Restriction Mapping,
pubmed-meshheading:9387877-Sequence Alignment,
pubmed-meshheading:9387877-Sequence Deletion,
pubmed-meshheading:9387877-TATA Box,
pubmed-meshheading:9387877-Transfection,
pubmed-meshheading:9387877-Uterus
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pubmed:year |
1997
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pubmed:articleTitle |
The mouse calretinin gene promoter region: structural and functional components.
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pubmed:affiliation |
NIMH, Laboratory of Clinical Science, Bethesda, MD 20892-1266, USA. kstrauss@thunder.ocis.temple.edu
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pubmed:publicationType |
Journal Article
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