| pubmed-article:9379509 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:9379509 | lifeskim:mentions | umls-concept:C0086418 | lld:lifeskim |
| pubmed-article:9379509 | lifeskim:mentions | umls-concept:C0010453 | lld:lifeskim |
| pubmed-article:9379509 | lifeskim:mentions | umls-concept:C0162638 | lld:lifeskim |
| pubmed-article:9379509 | lifeskim:mentions | umls-concept:C0007102 | lld:lifeskim |
| pubmed-article:9379509 | lifeskim:mentions | umls-concept:C0334227 | lld:lifeskim |
| pubmed-article:9379509 | lifeskim:mentions | umls-concept:C0002199 | lld:lifeskim |
| pubmed-article:9379509 | lifeskim:mentions | umls-concept:C0016360 | lld:lifeskim |
| pubmed-article:9379509 | lifeskim:mentions | umls-concept:C1456820 | lld:lifeskim |
| pubmed-article:9379509 | lifeskim:mentions | umls-concept:C0009429 | lld:lifeskim |
| pubmed-article:9379509 | lifeskim:mentions | umls-concept:C0205263 | lld:lifeskim |
| pubmed-article:9379509 | pubmed:issue | 4 | lld:pubmed |
| pubmed-article:9379509 | pubmed:dateCreated | 1997-11-10 | lld:pubmed |
| pubmed-article:9379509 | pubmed:abstractText | Biochemical analysis using nick end-labeling was performed to investigate the effect of various combinations of 5-fluorouracil, natural human tumor necrosis factor-alpha and natural human interferon-alpha on the induction of apoptosis in RPMI 4788 human colon cancer cells. After treatment with 5-fluorouracil (1 mM) for 48 h, the number of nick end-positive cells was significantly increased in comparison to the situation without treatment. When tumor cells were treated with 1 mM 5-fluorouracil, 2.86 Japan Reference Units (JRU)/ml natural human tumor necrosis factor-alpha and 1 x 10(3) IU/ml natural human interferon-alpha in combination for 48 h, the number of nick end-positive cells was significantly higher than that after treatment with 5-fluorouracil alone. MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) assay revealed a significant decrease of relative viability, as compared to treatment with 5-fluorouracil (1 mM), 5-fluorouracil + natural human tumor necrosis factor-alpha, or 5-fluorouracil + natural human interferon-alpha for 48 h. Pretreatment with 5-fluorouracil (1 mM) for 24 h prior to treatment with natural human tumor necrosis factor-alpha (2.86 JRU/ml) and natural human interferon-alpha (10(3) IU/ml) for 24 h resulted in a significant increase of nick end-positive cells compared to pretreatment with natural human tumor necrosis factor-alpha and natural human interferon-alpha prior to treatment with 5-fluorouracil for 24 h (p < 0.05). These results suggest that 5-fluorouracil alone can induce apoptosis in RPMI 4788 tumor cells and that this effect can be enhanced by combination with natural human tumor necrosis factor-alpha and natural human interferon-alpha. | lld:pubmed |
| pubmed-article:9379509 | pubmed:language | eng | lld:pubmed |
| pubmed-article:9379509 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9379509 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:9379509 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
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| pubmed-article:9379509 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9379509 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9379509 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9379509 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9379509 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9379509 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9379509 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9379509 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9379509 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:9379509 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:9379509 | pubmed:month | Aug | lld:pubmed |
| pubmed-article:9379509 | pubmed:issn | 0368-2811 | lld:pubmed |
| pubmed-article:9379509 | pubmed:author | pubmed-author:TanakaNN | lld:pubmed |
| pubmed-article:9379509 | pubmed:author | pubmed-author:HatanoHH | lld:pubmed |
| pubmed-article:9379509 | pubmed:author | pubmed-author:OkaYY | lld:pubmed |
| pubmed-article:9379509 | pubmed:author | pubmed-author:OritaKK | lld:pubmed |
| pubmed-article:9379509 | pubmed:author | pubmed-author:NaomotoYY | lld:pubmed |
| pubmed-article:9379509 | pubmed:author | pubmed-author:HaisaMM | lld:pubmed |
| pubmed-article:9379509 | pubmed:author | pubmed-author:YasuokaYY | lld:pubmed |
| pubmed-article:9379509 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:9379509 | pubmed:volume | 27 | lld:pubmed |
| pubmed-article:9379509 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:9379509 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:9379509 | pubmed:pagination | 231-5 | lld:pubmed |
| pubmed-article:9379509 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
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| pubmed-article:9379509 | pubmed:meshHeading | pubmed-meshheading:9379509-... | lld:pubmed |
| pubmed-article:9379509 | pubmed:year | 1997 | lld:pubmed |
| pubmed-article:9379509 | pubmed:articleTitle | Apoptosis in cultured human colon cancer cells induced by combined treatments with 5-fluorouracil, tumor necrosis factor-alpha and interferon-alpha. | lld:pubmed |
| pubmed-article:9379509 | pubmed:affiliation | First Department of Surgery, Okayama University Medical School, Japan. | lld:pubmed |
| pubmed-article:9379509 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:9379509 | pubmed:publicationType | Comparative Study | lld:pubmed |
| http://linkedlifedata.com/r... | pubmed:referesTo | pubmed-article:9379509 | lld:pubmed |
