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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
46
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pubmed:dateCreated |
1997-12-19
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pubmed:abstractText |
The mouse orphan nuclear receptor TR2-11-f suppressed the expression of reporters fused to a hormone response element of the mouse cellular retinoic acid-binding protein I gene promoter. TR2-11-f was able to bind to a direct repeat with four nucleotides in the spacer (5'TGACCTTTGGGGACCT3') located within this hormone response element as homodimers. The specificity of protein-DNA interactions was demonstrated by competition in gel retardation and antibody-mediated supershift reactions. The residues critical for TR2-11-f binding were mapped to both repeated sequences, whereas the spacer and the flanking sequences were less important. The Kd and Bmax of TR2-11-f homodimer binding to this direct repeat were determined to be 2.6 nM and 0.012 nM, respectively. By using a yeast two-hybrid system, it was demonstrated that dimerization of TR2-11-f was mediated by its ligand-binding domain. The actions of TR2-11-f in regulating cellular retinoic acid-binding protein I gene will likely influence retinoic action and availability within the cells.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Ligands,
http://linkedlifedata.com/resource/pubmed/chemical/Nr2c1 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Nuclear Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Nuclear Receptor Subfamily 2...,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Retinoic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Thyroid Hormone,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Tretinoin,
http://linkedlifedata.com/resource/pubmed/chemical/retinoic acid binding protein I...
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0006-2960
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
18
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pubmed:volume |
36
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
14088-95
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:9369481-Animals,
pubmed-meshheading:9369481-Base Sequence,
pubmed-meshheading:9369481-Binding, Competitive,
pubmed-meshheading:9369481-COS Cells,
pubmed-meshheading:9369481-DNA-Binding Proteins,
pubmed-meshheading:9369481-Dimerization,
pubmed-meshheading:9369481-Gene Expression Regulation,
pubmed-meshheading:9369481-Genes, Reporter,
pubmed-meshheading:9369481-Ligands,
pubmed-meshheading:9369481-Mice,
pubmed-meshheading:9369481-Molecular Sequence Data,
pubmed-meshheading:9369481-Nuclear Proteins,
pubmed-meshheading:9369481-Nuclear Receptor Subfamily 2, Group C, Member 1,
pubmed-meshheading:9369481-Promoter Regions, Genetic,
pubmed-meshheading:9369481-Protein Binding,
pubmed-meshheading:9369481-Receptors, Retinoic Acid,
pubmed-meshheading:9369481-Receptors, Thyroid Hormone,
pubmed-meshheading:9369481-Recombinant Proteins,
pubmed-meshheading:9369481-Saccharomyces cerevisiae,
pubmed-meshheading:9369481-Suppression, Genetic,
pubmed-meshheading:9369481-Tretinoin
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pubmed:year |
1997
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pubmed:articleTitle |
The orphan nuclear receptor TR2 suppresses a DR4 hormone response element of the mouse CRABP-I gene promoter.
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pubmed:affiliation |
Department of Pharmacology, University of Minnesota Medical School, Minneapolis, Minnesota 55455, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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