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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
10
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pubmed:dateCreated |
1997-12-10
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pubmed:abstractText |
HLA-G is a class Ib (non-classical) major histocompatibility complex (MHC) protein expressed at the maternal-fetal interface that inhibits natural killer (NK) cell-mediated lysis in an allotype-independent manner. Here we report that the spontaneous endocytosis of HLA-G is severely reduced because of its short cytoplasmic tail. Class I (classical) MHC proteins on the surface of B cell transfectants detected by primary and secondary antibodies underwent endocytosis at a moderate rate, whereas HLA-G, chimeric proteins consisting of the extracellular domains of HLA-C with the C-terminal sequence of HLA-G, or glycophosphatidylinositol-tailed HLA-C proteins, were not efficiently internalized. In addition, a mutant of beta 2-microglobulin (Ser88Cys) that could be specifically labeled with Texas red (or other fluorescent probes) and exchanged into class I or class Ib MHC proteins was employed to study spontaneous internalization of MHC proteins by a non-perturbative method independent of an antibody ligand. These data are discussed in terms of both the role of HLA-G expressed on the fetal trophoblast and the function of the cytoplasmic tail in class I MHC proteins.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0014-2980
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
27
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
2714-9
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:9368631-B-Lymphocytes,
pubmed-meshheading:9368631-Endocytosis,
pubmed-meshheading:9368631-Flow Cytometry,
pubmed-meshheading:9368631-Fluorescent Dyes,
pubmed-meshheading:9368631-HLA Antigens,
pubmed-meshheading:9368631-HLA-C Antigens,
pubmed-meshheading:9368631-HLA-G Antigens,
pubmed-meshheading:9368631-Histocompatibility Antigens Class I,
pubmed-meshheading:9368631-Humans,
pubmed-meshheading:9368631-Microscopy, Confocal,
pubmed-meshheading:9368631-Microscopy, Fluorescence,
pubmed-meshheading:9368631-Point Mutation,
pubmed-meshheading:9368631-Recombinant Fusion Proteins,
pubmed-meshheading:9368631-Structure-Activity Relationship,
pubmed-meshheading:9368631-Transfection,
pubmed-meshheading:9368631-Trophoblasts,
pubmed-meshheading:9368631-Xanthenes,
pubmed-meshheading:9368631-beta 2-Microglobulin
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pubmed:year |
1997
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pubmed:articleTitle |
Impaired spontaneous endocytosis of HLA-G.
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pubmed:affiliation |
Department of Molecular and Cellular Biology, Harvard University, Cambridge 02138, USA.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.
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