Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
47
|
| pubmed:dateCreated |
1997-12-23
|
| pubmed:abstractText |
The protein product of the c-fes proto-oncogene has been implicated in the normal development of myeloid cells (macrophages and granulocytes). We have previously shown that 151 base pairs of c-fes 5'-flanking sequences are sufficient for myeloid cell-specific expression and include functional binding sites for Sp1, PU.1, and a novel nuclear factor (Heydemann, A., Juang, G., Hennessy, K., Parmacek, M. S., and Simon, M. C. (1996) Mol. Cell. Biol. 16, 1676-1686). This novel hematopoietic transcription factor, termed FEF (c-fes expression factor), binds to a cis-acting element that is located at nucleotides -9 to -4 of the c-fes promoter between two Ets binding sites (at -19 to -15 and -4 to +1) which bind PU.1. We now show that a FEF binding site exists in the myeloid cell-specific regulatory region of a second gene, the -2.7-kilobase pair enhancer of chicken lysozyme. The lysozyme FEF site is immediately 5' to a PU. 1 site, analogous to their arrangement in the c-fes promoter, and allows the formation of a preliminary FEF consensus site, 5'-GAAT(C/G)A-3'. This consensus site does not match any sites for known transcription factors. Importantly, although PU.1 binds immediately 3' of the FEF site in both the c-fes promoter and the chicken lysozyme enhancer (CLE), we show that they bind independently. The FEF sites are required for high levels of transcription by both the CLE and the c-fes promoter in transient transfection experiments. Importantly, elimination of the CLE FEF site abolishes all transcriptional activity of this enhancer element. Mutation of the adjacent PU.1 site in either the c-fes promoter or the CLE, reduces activity by approximately 50%. Therefore, transcription of both lysozyme and fes in myeloid cells requires FEF and PU.1. UV cross-linking experiments show that the FEF binding activity consists of a single 70-kDa protein in both human and murine cell lines. FEF binding activity is not affected by antibodies that specifically recognize a number of cloned transcription factors. Collectively, these data indicate that we have identified a novel transcription factor that is functionally important for the expression of at least two myeloid cell-specific genes.
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| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/FES protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Fes protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Muramidase,
http://linkedlifedata.com/resource/pubmed/chemical/Protein-Tyrosine Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-fes,
http://linkedlifedata.com/resource/pubmed/chemical/Trans-Activators,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/proto-oncogene protein Spi-1
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Nov
|
| pubmed:issn |
0021-9258
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
21
|
| pubmed:volume |
272
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
29527-37
|
| pubmed:dateRevised |
2009-11-19
|
| pubmed:meshHeading |
pubmed-meshheading:9368014-Animals,
pubmed-meshheading:9368014-Base Sequence,
pubmed-meshheading:9368014-Binding Sites,
pubmed-meshheading:9368014-Consensus Sequence,
pubmed-meshheading:9368014-DNA,
pubmed-meshheading:9368014-DNA-Binding Proteins,
pubmed-meshheading:9368014-Hematopoietic Stem Cells,
pubmed-meshheading:9368014-Humans,
pubmed-meshheading:9368014-Membrane Proteins,
pubmed-meshheading:9368014-Mice,
pubmed-meshheading:9368014-Molecular Sequence Data,
pubmed-meshheading:9368014-Molecular Weight,
pubmed-meshheading:9368014-Muramidase,
pubmed-meshheading:9368014-Promoter Regions, Genetic,
pubmed-meshheading:9368014-Protein-Tyrosine Kinases,
pubmed-meshheading:9368014-Proto-Oncogene Proteins,
pubmed-meshheading:9368014-Proto-Oncogene Proteins c-fes,
pubmed-meshheading:9368014-Trans-Activators,
pubmed-meshheading:9368014-Transcription, Genetic,
pubmed-meshheading:9368014-Transcription Factors,
pubmed-meshheading:9368014-Tumor Cells, Cultured
|
| pubmed:year |
1997
|
| pubmed:articleTitle |
Expression of two myeloid cell-specific genes requires the novel transcription factor, c-fes expression factor.
|
| pubmed:affiliation |
Department of Molecular Genetics and Cell Biology, University of Chicago, Chicago, Illinois 60637, USA.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|