Linked Life Data

9366896

Source:http://linkedlifedata.com/resource/pubmed/id/9366896

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1997-12-8
pubmed:abstractText
The potent bacterial mutagen 3-chloro-4-(dichloromethyl)-5-hydroxy-2[5H]-furanone (MX), which is formed during chlorination of drinking water, has been studied with respect to induction of cell death in promyelocytic leukemic HL-60 cells. Cells exposed to MX for 1 h and further incubated for 3 h, revealed no significant increase in the proportion of cells with compromised plasma membrane damage as judged by trypan blue or propidium iodide exclusion. However, flow cytometric studies and microscopic analysis of HL-60 cells after staining with Giemsa or Hoechst 33342, revealed that more than 30% of the cells exposed to 30-100 microM of MX, showed the characteristic morphology and biochemical markers of apoptosis. On the other hand, in cultures exposed to 300 microM MX, less than 5% of the cells appeared to be apoptotic (< G1 DNA) 3 h after treatment, which is similar to control values. Microscopic analysis of Hoechst 33342-stained cells revealed that they were 'arrested' in the early stages of chromatin condensation, but these cells eventually became necrotic. Some decrease in the percentage of cells in S-phase was observed 3 h after exposure to MX (10, 30 and 100 microM), but the induced cell death was not markedly cell stage specific. The characteristic ladder pattern of apoptotic cells was observed when DNA isolated from MX-exposed HL-60 cells was electrophoresed in agarose. The apoptotic process could also be detected by analysis with alkaline filter elution (AE), as a decrease in the total DNA recovered; and by single cell gel electrophoresis, as a decrease in the average number of cells/comets observable on each slide. With the protocols used no apparent increase in values in the normalized area above the curve (NAAC) (alkaline elution) or tail moments (single cell gel electrophoresis (SCGE)) were detected, indicating that apoptotic cells are not necessarily a confounding factor when assaying for genotoxicity with these techniques.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0009-2797
pubmed:author
pubmed:issnType
Print
pubmed:day
12
pubmed:volume
106
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
89-107
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:9366896-Adult, pubmed-meshheading:9366896-Apoptosis, pubmed-meshheading:9366896-Azure Stains, pubmed-meshheading:9366896-Benzimidazoles, pubmed-meshheading:9366896-Cell Cycle, pubmed-meshheading:9366896-Cell Membrane, pubmed-meshheading:9366896-Cell Size, pubmed-meshheading:9366896-Cell Survival, pubmed-meshheading:9366896-DNA Damage, pubmed-meshheading:9366896-DNA Fragmentation, pubmed-meshheading:9366896-Electrophoresis, Agar Gel, pubmed-meshheading:9366896-Female, pubmed-meshheading:9366896-Flow Cytometry, pubmed-meshheading:9366896-Furans, pubmed-meshheading:9366896-HL-60 Cells, pubmed-meshheading:9366896-Histocytochemistry, pubmed-meshheading:9366896-Humans, pubmed-meshheading:9366896-Mutagens, pubmed-meshheading:9366896-Propidium, pubmed-meshheading:9366896-Trypan Blue
pubmed:year
1997
pubmed:articleTitle
Apoptosis in HL-60 cells induced by 3-chloro-4-(dichloromethyl)-5-hydroxy-2[5H]-furanone (MX).
pubmed:affiliation
Department of Environmental Medicine, National Institute of Public Health, Oslo, Norway.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't