Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
8
|
| pubmed:dateCreated |
1998-1-30
|
| pubmed:abstractText |
Two site-specific endonucleases, RspLKI and RspLKII, have been isolated and purified to functional homogeneity from the soil bacterium Rhodococcus species LK2. RspLKI recognizes the 5'-GCATG decreases C-3' DNA sequence and RspLKII recognizes the 5'-G decreases GATCC-3' sequence (arrows indicate DNA cleavage sites). The isolated enzymes are class II site specific endonucleases and are isoschizomers of endonucleases SphI and BamHI, respectively.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Aug
|
| pubmed:issn |
0006-2979
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
62
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
872-82
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:9360299-Chromatography, Ion Exchange,
pubmed-meshheading:9360299-DNA,
pubmed-meshheading:9360299-Deoxyribonuclease BamHI,
pubmed-meshheading:9360299-Deoxyribonucleases, Type II Site-Specific,
pubmed-meshheading:9360299-Electrophoresis, Agar Gel,
pubmed-meshheading:9360299-Rhodococcus equi,
pubmed-meshheading:9360299-Substrate Specificity
|
| pubmed:year |
1997
|
| pubmed:articleTitle |
Site-specific endonucleases RspLKI and RspLKII from Rhodococcus species LK2 are isoschizomers of SphI and BamHI.
|
| pubmed:affiliation |
Institute of Protein Research, Russian Academy of Sciences, Pushchino, Moscow Region, Russia. protres@sovam.com
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|