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rdf:type |
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lifeskim:mentions |
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pubmed:dateCreated |
1997-12-2
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pubmed:abstractText |
A lipoxygenase inhibitor in the cytosolic fraction of human epidermoid carcinoma A431 cells was characterized and purified. The cytosolic inhibitor lost the inhibitory activity upon heating at 75 degrees C for 15 min or pretreating with 1 mg/ml trypsin at 37 degrees C for 60 min. Cytosol, after dialysis, lost the inhibitory activity but its inhibitory activity recovered when 1 mM GSH was added to the dialysate. The inhibitory activity of cytosol was also abolished by treatment either with 1 mM iodoacetate at 4 degrees C for 1 h or with 0.5 mM H2O2. The pI of the inhibitor was approx. 7.0. In addition to 12-lipoxygenase, the inhibitor inhibited the activities of 5-lipoxygenase and fatty acid cyclo-oxygenase in a cell-free system. The inhibitor was purified by a series of column chromatographies using CM Sephadex C-50, Sephadex G-100 SF and Mono P columns. A major 22 kDa protein was obtained that was distinct from selenium-dependent glutathione peroxidase.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/9355752-1125011,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9355752-14907713,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9355752-164225,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9355752-1740418,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9355752-1854807,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9355752-1901823,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9355752-1903304,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9355752-2037564,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9355752-2828053,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9355752-3038886,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9355752-3087141,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9355752-3194911,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9355752-4058268,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9355752-4203739,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9355752-4373005,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/9355752-5432063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9355752-6162199,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9355752-6807297,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9355752-7236659,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9355752-7378432,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9355752-7511046,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9355752-7738031,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9355752-7868854,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9355752-8051068,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/9355752-9022753
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0264-6021
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
327 ( Pt 1)
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
193-8
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:9355752-Arachidonic Acid,
pubmed-meshheading:9355752-Chromatography, Gel,
pubmed-meshheading:9355752-Chromatography, Ion Exchange,
pubmed-meshheading:9355752-Cytosol,
pubmed-meshheading:9355752-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:9355752-Enzyme Stability,
pubmed-meshheading:9355752-Glutathione,
pubmed-meshheading:9355752-Humans,
pubmed-meshheading:9355752-Iodoacetates,
pubmed-meshheading:9355752-Iodoacetic Acid,
pubmed-meshheading:9355752-Isoelectric Point,
pubmed-meshheading:9355752-Lipoxygenase,
pubmed-meshheading:9355752-Lipoxygenase Inhibitors,
pubmed-meshheading:9355752-Microsomes,
pubmed-meshheading:9355752-Molecular Weight,
pubmed-meshheading:9355752-Temperature,
pubmed-meshheading:9355752-Tumor Cells, Cultured
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pubmed:year |
1997
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pubmed:articleTitle |
Characterization and purification of a lipoxygenase inhibitor in human epidermoid carcinoma A431 cells.
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pubmed:affiliation |
Department of Pharmacology, College of Medicine, National Cheng Kung University, Tainan, Taiwan 70101, Republic of China.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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