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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
11
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pubmed:dateCreated |
1997-11-13
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pubmed:abstractText |
It has been reported previously that immunization with recombinant protein containing the two epidermal growth factor (EGF)-like modules from merozoite surface protein 1 (MSP-1) of Plasmodium yoelii (strain YM) protects mice against a lethal blood-stage challenge with the same parasite strain. Since MSP-1 is expressed in both liver- and blood-stage schizonts and on the surface of merozoites, we evaluated the effectiveness of immunization with recombinant proteins containing either the individual or the two combined EGF-like modules in producing a protective response against a sporozoite challenge. The recombinant protein expressing the combined EGF-like modules of the YM strain protected mice against a homologous sporozoite challenge, and sterile protection, as defined by the absence of detectable blood-stage parasites, was observed in the majority of the mice. In contrast, mice immunized with recombinant P. yoelii YM MSP-1 were not protected against a heterologous challenge with sporozoites from strain 265 BY of P. yoelii. The lack of protection may be explained by differences identified in the amino acid sequences of MSP-1 for the two strains. A recombinant protein containing the two EGF-like modules of MSP-1 from P. yoelii 265 BY was produced and used to immunize mice. These mice were protected against a homologous challenge with sporozoites of P. yoelii 265 BY. The results suggest that a recombinant MSP-1 has potential as a vaccine against malaria, but its efficacy may be limited by sequence polymorphism and selection of variants.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/9353014-1381314,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9353014-15275468,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9353014-15463703,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9353014-1620166,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9353014-1624802,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9353014-1625908,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9353014-1775158,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9353014-1878339,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9353014-1980910,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9353014-1991487,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9353014-2052407,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9353014-2201546,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9353014-2258697,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9353014-2358778,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9353014-2447163,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9353014-2467942,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9353014-2469336,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9353014-2477452,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9353014-2509909,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9353014-2797063,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9353014-2871847,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9353014-3085218,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9353014-3275737,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9353014-3308506,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/9353014-7516416,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9353014-7602100,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9353014-7628566,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9353014-7718511,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9353014-8015856,
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http://linkedlifedata.com/resource/pubmed/commentcorrection/9353014-8458376,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9353014-8515786,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9353014-8577318,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9353014-8617331,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9353014-8627050,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9353014-8751907
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0019-9567
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
65
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
4419-23
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:9353014-Amino Acid Sequence,
pubmed-meshheading:9353014-Animals,
pubmed-meshheading:9353014-Antigens, Protozoan,
pubmed-meshheading:9353014-Female,
pubmed-meshheading:9353014-Immunization,
pubmed-meshheading:9353014-Malaria,
pubmed-meshheading:9353014-Malaria Vaccines,
pubmed-meshheading:9353014-Merozoite Surface Protein 1,
pubmed-meshheading:9353014-Mice,
pubmed-meshheading:9353014-Mice, Inbred BALB C,
pubmed-meshheading:9353014-Molecular Sequence Data,
pubmed-meshheading:9353014-Peptide Fragments,
pubmed-meshheading:9353014-Plasmodium yoelii,
pubmed-meshheading:9353014-Protein Precursors,
pubmed-meshheading:9353014-Protozoan Proteins
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pubmed:year |
1997
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pubmed:articleTitle |
Immunization with a recombinant C-terminal fragment of Plasmodium yoelii merozoite surface protein 1 protects mice against homologous but not heterologous P. yoelii sporozoite challenge.
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pubmed:affiliation |
U313 INSERM, CHU Pitié-Salpêtrière, Paris, France. renia@icgm.cochin.inserm.fr
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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