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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
1997-11-20
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pubmed:abstractText |
We have previously demonstrated that corticotrophin-releasing factor receptor 1 (CRF-R1) mRNA levels can be down-regulated via activation of the cyclic AMP pathway in CATH.a cells, a neuronal cell line. In this study, we show evidence for down-regulation of CRF-R1 mRNA levels via activation of the protein kinase C (PKC) and calcium second messenger pathways. Incubation of CATH.a cells with phorbol 12-myristate 13-acetate (PMA), an activator of PKC, resulted in a time- and concentration-dependent down-regulation of CRF-R1 mRNA levels. Pretreatment with the inactive phorbol ester 4alpha-phorbol failed to influence significantly CRF-R1 mRNA levels. Incubation with carbachol, a cholinergic agonist known to activate PKC and increase intracellular calcium levels via phosphatidylinositol breakdown, also down-regulated CRF-R1 mRNA levels. Intracellular calcium levels were directly increased using A23187, a calcium ionophore, and thapsigargin, a calcium-ATPase inhibitor. Elevation of intracellular calcium content using either A23187 or thapsigargin significantly down-regulated levels of CRF-R1 mRNA. Furthermore, chelation of calcium with EGTA or blockade of voltage-dependent calcium channels with nifedipine inhibited agonist-mediated down-regulation of CRF-R1 mRNA levels. These results indicate that activation of PKC or calcium signal transduction pathways is sufficient to cause down-regulation of CRF-R1 mRNA levels and that calcium is required for agonist-mediated down-regulation of this receptor.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Alkaloids,
http://linkedlifedata.com/resource/pubmed/chemical/Benzophenanthridines,
http://linkedlifedata.com/resource/pubmed/chemical/CRF receptor type 1,
http://linkedlifedata.com/resource/pubmed/chemical/Calcimycin,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/Carbachol,
http://linkedlifedata.com/resource/pubmed/chemical/Corticotropin-Releasing Hormone,
http://linkedlifedata.com/resource/pubmed/chemical/Egtazic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/Enzyme Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Nifedipine,
http://linkedlifedata.com/resource/pubmed/chemical/Phenanthridines,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Kinase C,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Corticotropin-Releasing...,
http://linkedlifedata.com/resource/pubmed/chemical/Tetradecanoylphorbol Acetate,
http://linkedlifedata.com/resource/pubmed/chemical/Thapsigargin,
http://linkedlifedata.com/resource/pubmed/chemical/chelerythrine
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0022-3042
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
69
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1912-9
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:9349535-Alkaloids,
pubmed-meshheading:9349535-Animals,
pubmed-meshheading:9349535-Benzophenanthridines,
pubmed-meshheading:9349535-Calcimycin,
pubmed-meshheading:9349535-Calcium,
pubmed-meshheading:9349535-Carbachol,
pubmed-meshheading:9349535-Cell Line,
pubmed-meshheading:9349535-Corticotropin-Releasing Hormone,
pubmed-meshheading:9349535-Down-Regulation,
pubmed-meshheading:9349535-Egtazic Acid,
pubmed-meshheading:9349535-Enzyme Inhibitors,
pubmed-meshheading:9349535-Kinetics,
pubmed-meshheading:9349535-Mice,
pubmed-meshheading:9349535-Nifedipine,
pubmed-meshheading:9349535-Phenanthridines,
pubmed-meshheading:9349535-Protein Kinase C,
pubmed-meshheading:9349535-RNA, Messenger,
pubmed-meshheading:9349535-Receptors, Corticotropin-Releasing Hormone,
pubmed-meshheading:9349535-Tetradecanoylphorbol Acetate,
pubmed-meshheading:9349535-Thapsigargin,
pubmed-meshheading:9349535-Transcription, Genetic
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pubmed:year |
1997
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pubmed:articleTitle |
Phorbol ester and calcium regulation of corticotrophin-releasing factor receptor 1 expression in a neuronal cell line.
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pubmed:affiliation |
Department of Psychiatry, Connecticut Mental Health Center, Yale University School of Medicine, New Haven 06508, U.S.A.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.
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