Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
9
|
| pubmed:dateCreated |
1997-11-18
|
| pubmed:abstractText |
The extracellular matrix produced by stromal cells plays a critical role in lympho-hematopoiesis. It was recently discovered that matrix glycoprotein SC1/ECM2 is a component of that matrix and preliminary evidence suggested that it could contribute to the nurturing environment for B-lymphocyte precursors. A fusion protein prepared from the amino terminal portion of SC1/ECM2 and the constant region of human Ig preferentially bound to pre-B cells. Furthermore, the cloning efficiency of interleukin-7-dependent B-cell precursors was increased in a dose-dependent manner by addition of this fusion protein. We now report the complete cDNA sequence for murine SC1/ECM2 and its localization to the central region of chromosome 5. A fusion protein prepared from the full length of SC1/ECM2 and Ig was found to recognize pre-B cells in a divalent cation-dependent manner, and to augment mitogen-dependent proliferation of mature B cells, as well as the cloning of pre-B cells, but to have no influence on myeloid progenitor cells. Although SC1/ECM2 is normally a secreted protein, we show that it is also capable of augmenting lymphopoiesis when expressed as a transmembrane protein on fibroblasts. Although the C-terminal portion of SC1/ECM2 has sequence homology to osteonectin/SPARC, the unique N-terminal one fifth of the protein was sufficient to augment lymphocyte growth.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
AIM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Activated-Leukocyte Cell Adhesion...,
http://linkedlifedata.com/resource/pubmed/chemical/Extracellular Matrix Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulins,
http://linkedlifedata.com/resource/pubmed/chemical/Nerve Tissue Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Nov
|
| pubmed:issn |
0006-4971
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
1
|
| pubmed:volume |
90
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
3404-13
|
| pubmed:dateRevised |
2007-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:9345023-Activated-Leukocyte Cell Adhesion Molecule,
pubmed-meshheading:9345023-Amino Acid Sequence,
pubmed-meshheading:9345023-Animals,
pubmed-meshheading:9345023-B-Lymphocytes,
pubmed-meshheading:9345023-Cell Differentiation,
pubmed-meshheading:9345023-Chromosome Mapping,
pubmed-meshheading:9345023-Cloning, Molecular,
pubmed-meshheading:9345023-Extracellular Matrix Proteins,
pubmed-meshheading:9345023-Hematopoiesis,
pubmed-meshheading:9345023-Humans,
pubmed-meshheading:9345023-Immunoglobulins,
pubmed-meshheading:9345023-Mice,
pubmed-meshheading:9345023-Molecular Sequence Data,
pubmed-meshheading:9345023-Nerve Tissue Proteins,
pubmed-meshheading:9345023-Rats,
pubmed-meshheading:9345023-Recombinant Fusion Proteins,
pubmed-meshheading:9345023-Sequence Alignment,
pubmed-meshheading:9345023-Stromal Cells
|
| pubmed:year |
1997
|
| pubmed:articleTitle |
Matrix glycoprotein SC1/ECM2 augments B lymphopoiesis.
|
| pubmed:affiliation |
Oklahoma Medical Research Foundation, Oklahoma City, Oklahoma, USA.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
|