Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1998-1-6
pubmed:abstractText
We recently described a stimulatory effect of high doses (> 100 mumol/L) diclofenac on platelet adhesion. In this study we extend our research to the possible biochemical mechanisms of the observed effects, to other non steroidal anti-inflammatory drugs (NSAIDs) (flurbiprofen, indomethacin, acetylsalicylic acid, ibuprofen, nitrofenac and nitroflurbiprofen) and to the effect of high doses diclofenac and flurbiprofen on platelet aggregation. We observed that high doses of diclofenac and of flurbiprofen, but not of the other tested NSAIDs, increased platelet adhesion at doses ranging from 100 to 500 mumol/L, an effect completely removed by the 12-lipoxygenase-inhibitor nordihydroguaiaretic acid. Moreover, they had no pro-aggregating effect, inhibiting platelet aggregation induced by 10 mumol/L arachidonic acid and dose-dependently increasing the [Ca2+]i. Finally, whereas no basal nitric oxide release by washed platelets was detected, when platelets were incubated by 500 mumol/L diclofenac or flurbiprofen, the production of nitric oxide, as measured by amounts of nitrite released, was 4.4 +/- 0.5 and 3.8 +/- 0.4 pmol/5 x 10(8) platelets/min, respectively. Our data indicate that high doses diclofenac and flurbiprofen are promoters of the early phases of platelet activation, probably through the 12-lipoxygenase pathway.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/Anti-Inflammatory Agents..., http://linkedlifedata.com/resource/pubmed/chemical/Arachidonate 12-Lipoxygenase, http://linkedlifedata.com/resource/pubmed/chemical/Aristolochic Acids, http://linkedlifedata.com/resource/pubmed/chemical/Calcium, http://linkedlifedata.com/resource/pubmed/chemical/Diclofenac, http://linkedlifedata.com/resource/pubmed/chemical/Enzyme Inhibitors, http://linkedlifedata.com/resource/pubmed/chemical/Flurbiprofen, http://linkedlifedata.com/resource/pubmed/chemical/Lipoxygenase Inhibitors, http://linkedlifedata.com/resource/pubmed/chemical/Nitric Oxide, http://linkedlifedata.com/resource/pubmed/chemical/Nordihydroguaiaretic Acid, http://linkedlifedata.com/resource/pubmed/chemical/Phenanthrenes, http://linkedlifedata.com/resource/pubmed/chemical/Phospholipases A, http://linkedlifedata.com/resource/pubmed/chemical/aristolochic acid I
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0360-3997
pubmed:author
pubmed:issnType
Print
pubmed:volume
21
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
519-30
pubmed:dateRevised
2010-11-18
pubmed:meshHeading
pubmed-meshheading:9343749-Anti-Inflammatory Agents, Non-Steroidal, pubmed-meshheading:9343749-Arachidonate 12-Lipoxygenase, pubmed-meshheading:9343749-Aristolochic Acids, pubmed-meshheading:9343749-Calcium, pubmed-meshheading:9343749-Diclofenac, pubmed-meshheading:9343749-Dose-Response Relationship, Drug, pubmed-meshheading:9343749-Enzyme Inhibitors, pubmed-meshheading:9343749-Flurbiprofen, pubmed-meshheading:9343749-Humans, pubmed-meshheading:9343749-Inflammation, pubmed-meshheading:9343749-Lipoxygenase Inhibitors, pubmed-meshheading:9343749-Nitric Oxide, pubmed-meshheading:9343749-Nordihydroguaiaretic Acid, pubmed-meshheading:9343749-Phenanthrenes, pubmed-meshheading:9343749-Phospholipases A, pubmed-meshheading:9343749-Platelet Activation, pubmed-meshheading:9343749-Platelet Adhesiveness, pubmed-meshheading:9343749-Platelet Aggregation
pubmed:year
1997
pubmed:articleTitle
Study on paradoxical effects of NSAIDs on platelet activation.
pubmed:affiliation
Institute of Clinical Chemistry, University of Verona, Italy.
pubmed:publicationType
Journal Article, In Vitro, Research Support, Non-U.S. Gov't