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rdf:type |
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lifeskim:mentions |
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pubmed:dateCreated |
1997-11-13
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pubmed:abstractText |
Recently a 25-residue part of Gag polyprotein from HIV type 1 (HIV-1) was reported to bind to the cytosolic 18 kDa cyclophilin (Cyp18) with an IC50 value of 180 microM. This peptide corresponds to the Cyp18-binding domain of HIV-1 Gag. A replacement of Gly with Ala in the cyclophilin-binding loop of HIV-1 Gag polyprotein results in the prevention of the packaging of Cyp18 into virions. We found only two conformers of this peptide among 16 possible expected conformers, owing to cis/trans isomerization of four peptidyl-prolyl bonds. Although this finding implicates the existence of a stabilizing structure, we were not able to detect secondary structure formation by 1H-NMR and CD spectroscopy. We characterized the peptide as a substrate for Cyp18 by two-dimensional exchange 1H-NMR spectroscopy. Surprisingly, we found similar binding characteristics for a peptide corresponding to 25-mer peptide containing the above-mentioned Gly to Ala substitution.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/9337866-1548743,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9337866-2186809,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9337866-2417625,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9337866-28142,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9337866-2843644,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9337866-3184187,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9337866-3306408,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9337866-3741839,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9337866-6395866,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9337866-7275014,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9337866-7538221,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9337866-7540055,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9337866-7881270,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9337866-7957056,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9337866-7969494,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9337866-7969495,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9337866-8019135,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9337866-8155653,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9337866-8513493,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9337866-8645165,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9337866-8648689,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9337866-8662505,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9337866-8676442,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9337866-8676452,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9337866-8692877,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9337866-8784350,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9337866-8830660,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9337866-8871396,
http://linkedlifedata.com/resource/pubmed/commentcorrection/9337866-9016720
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0264-6021
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
326 ( Pt 1)
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
181-5
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:9337866-Amino Acid Sequence,
pubmed-meshheading:9337866-Capsid,
pubmed-meshheading:9337866-HIV Core Protein p24,
pubmed-meshheading:9337866-Humans,
pubmed-meshheading:9337866-Magnetic Resonance Spectroscopy,
pubmed-meshheading:9337866-Methionine,
pubmed-meshheading:9337866-Molecular Sequence Data,
pubmed-meshheading:9337866-Norleucine,
pubmed-meshheading:9337866-Peptide Fragments,
pubmed-meshheading:9337866-Peptidylprolyl Isomerase,
pubmed-meshheading:9337866-Protein Binding,
pubmed-meshheading:9337866-Protein Conformation,
pubmed-meshheading:9337866-Solutions
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pubmed:year |
1997
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pubmed:articleTitle |
Conformational state of a 25-mer peptide from the cyclophilin-binding loop of the HIV type 1 capsid protein.
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pubmed:affiliation |
Max-Planck-Gesellschaft, Forschungsstelle Enzymologie der Proteinfaltung, Halle/S., Federal Republic of Germany.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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