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rdf:type |
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lifeskim:mentions |
umls-concept:C0007634,
umls-concept:C0017262,
umls-concept:C0035820,
umls-concept:C0075233,
umls-concept:C0079281,
umls-concept:C0086418,
umls-concept:C0178719,
umls-concept:C0185117,
umls-concept:C0206686,
umls-concept:C0596235,
umls-concept:C0851285,
umls-concept:C2911684
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pubmed:issue |
10
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pubmed:dateCreated |
1997-10-23
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pubmed:abstractText |
The role played by endothelin (ET-1) and its receptor subtypes A and B (ET(A) and ET(B)) in the functional regulation of human NCI-H295 adrenocortical carcinoma cells has been investigated. Reverse transcription-PCR with primers specific for prepro-ET-1, human ET-1 converting enzyme-1, ET(A), and ET(B) complementary DNAs consistently demonstrated the expression of all genes in NCI-H295 cells. The presence of mature ET-1 and both its receptor subtypes was confirmed by immunocytochemistry and autoradiography, respectively. Aldosterone synthase (AS) messenger RNA was also detected in NCI-H295 cells, and AS gene expression was enhanced by both ET-1 and the specific ET(B) agonist IRL-1620; this effect was not inhibited by either the ET(A) antagonist BQ-123 or the ET(B) antagonist BQ-788. A clear-cut increase in the intracellular Ca2+ concentration in NCI-H295 cells in response to ET(B), but not ET(A), activation was observed. In light of these findings, the following conclusions can be drawn: 1) NCI-H295 cells possess an active ET-1 biosynthetic pathway and are provided with ET(A) and ET(B) receptors; 2) ET-1 regulates in an autocrine/paracrine fashion the secretion of aldosterone by NCI-H295 cells by enhancing both AS transcription and raising the intracellular Ca2+ concentration; and 3) the former effect of ET-1 probably involves the activation of both receptor subtypes, whereas calcium response is exclusively mediated by the ET(B) receptor.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Aldosterone Synthase,
http://linkedlifedata.com/resource/pubmed/chemical/Aspartic Acid Endopeptidases,
http://linkedlifedata.com/resource/pubmed/chemical/BQ 788,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Complementary,
http://linkedlifedata.com/resource/pubmed/chemical/Endothelin-1,
http://linkedlifedata.com/resource/pubmed/chemical/Endothelins,
http://linkedlifedata.com/resource/pubmed/chemical/Metalloendopeptidases,
http://linkedlifedata.com/resource/pubmed/chemical/Oligopeptides,
http://linkedlifedata.com/resource/pubmed/chemical/Piperidines,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Precursors,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Endothelin,
http://linkedlifedata.com/resource/pubmed/chemical/endothelin-converting enzyme
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0013-7227
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
138
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
4421-6
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:9322959-Adrenal Cortex Neoplasms,
pubmed-meshheading:9322959-Adrenocortical Carcinoma,
pubmed-meshheading:9322959-Aldosterone Synthase,
pubmed-meshheading:9322959-Aspartic Acid Endopeptidases,
pubmed-meshheading:9322959-Autoradiography,
pubmed-meshheading:9322959-Base Sequence,
pubmed-meshheading:9322959-Calcium,
pubmed-meshheading:9322959-DNA, Complementary,
pubmed-meshheading:9322959-Endothelin-1,
pubmed-meshheading:9322959-Endothelins,
pubmed-meshheading:9322959-Gene Expression Regulation, Enzymologic,
pubmed-meshheading:9322959-Humans,
pubmed-meshheading:9322959-Immunohistochemistry,
pubmed-meshheading:9322959-Metalloendopeptidases,
pubmed-meshheading:9322959-Oligopeptides,
pubmed-meshheading:9322959-Piperidines,
pubmed-meshheading:9322959-Polymerase Chain Reaction,
pubmed-meshheading:9322959-Protein Precursors,
pubmed-meshheading:9322959-Receptors, Endothelin,
pubmed-meshheading:9322959-Tumor Cells, Cultured
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pubmed:year |
1997
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pubmed:articleTitle |
Autocrine-paracrine role of endothelin-1 in the regulation of aldosterone synthase expression and intracellular Ca2+ in human adrenocortical carcinoma NCI-H295 cells.
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pubmed:affiliation |
Department of Clinical and Experimental Medicine, University of Padua Medical School, Padova, Italy. gprossi@ipdunidx.unipd.it
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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