Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
7
|
| pubmed:dateCreated |
1997-10-21
|
| pubmed:abstractText |
We previously reported that resting mouse peritoneal macrophages (PM) constitutively express low levels of IFN-gamma, whose production is consistently enhanced by exogenous IFN-gamma. In this study, we investigated the effects of IL-12 on the replication of vesicular stomatitis virus and on IFN-gamma gene expression in mouse PM. The addition of IL-12 to freshly explanted PM resulted in the persistence of an antiviral state to vesicular stomatitis virus, while control PM progressively became permissive for virus replication after 3 to 4 days in culture. The IL-12-induced antiviral state was inhibited by Abs to IFN-gamma, suggesting that endogenous IFN-gamma was largely responsible for this antiviral response. Moreover, IL-12 induced a consistent secretion of IFN-gamma, especially in cultured PM. The IL-1 2-induced antiviral state and IFN-gamma production were observed using PM from various strains of mice, including LPS-defective C3H/HeJ, NK-deficient bg/bg, DBA/2, Swiss (CD1), and Swiss nude mice treated or not with anti-asialo GM1 Abs. A 4-h treatment with IL-12 was sufficient to induce a marked accumulation of IFN-gamma mRNA, which was greater in cultured PM than in freshly harvested cells. Lastly, immunofluorescence studies in IL-12-stimulated macrophages clearly showed an enhancement of immunoreactive IFN-gamma compared with basal levels in cells exhibiting a macrophage (i.e., F4/80-positive) phenotype. Together, these findings demonstrate that IL-12 can directly stimulate mouse PM to produce IFN-gamma. We suggest that IL-12-induced IFN-gamma production by macrophages can play some role in the generation of the antiviral and immunoregulatory effects of IL-12.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
AIM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Differentiation,
http://linkedlifedata.com/resource/pubmed/chemical/Antiviral Agents,
http://linkedlifedata.com/resource/pubmed/chemical/G(M1) Ganglioside,
http://linkedlifedata.com/resource/pubmed/chemical/Interferon-gamma,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-12,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/asialo GM1 ganglioside,
http://linkedlifedata.com/resource/pubmed/chemical/monocyte-macrophage...
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Oct
|
| pubmed:issn |
0022-1767
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
1
|
| pubmed:volume |
159
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
3490-7
|
| pubmed:dateRevised |
2008-11-21
|
| pubmed:meshHeading |
pubmed-meshheading:9317148-Animals,
pubmed-meshheading:9317148-Antibodies,
pubmed-meshheading:9317148-Antigens, Differentiation,
pubmed-meshheading:9317148-Antiviral Agents,
pubmed-meshheading:9317148-Cells, Cultured,
pubmed-meshheading:9317148-G(M1) Ganglioside,
pubmed-meshheading:9317148-Interferon-gamma,
pubmed-meshheading:9317148-Interleukin-12,
pubmed-meshheading:9317148-Intracellular Fluid,
pubmed-meshheading:9317148-Macrophages, Peritoneal,
pubmed-meshheading:9317148-Male,
pubmed-meshheading:9317148-Mice,
pubmed-meshheading:9317148-Mice, Inbred C3H,
pubmed-meshheading:9317148-Mice, Inbred DBA,
pubmed-meshheading:9317148-Mice, Nude,
pubmed-meshheading:9317148-RNA, Messenger,
pubmed-meshheading:9317148-Species Specificity,
pubmed-meshheading:9317148-Tumor Cells, Cultured,
pubmed-meshheading:9317148-Vesicular stomatitis Indiana virus
|
| pubmed:year |
1997
|
| pubmed:articleTitle |
IL-12 induces IFN-gamma expression and secretion in mouse peritoneal macrophages.
|
| pubmed:affiliation |
Laboratorie of Immunology, Istituto Superiore di Sanità, Rome, Italy.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|